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Brock Biology of Microorganisms Chapter 12 Exam Study Guide. CA$15.08   Add to cart
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Brock Biology of Microorganisms Chapter 12 Exam Study Guide.
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Brock Biology of Microorganisms Chapter 12 Exam Study Guide. PCR (Polymerase Chain Reaction) - answerMultiplying segments of DNA up to a billionfold during amplification Thermocycler - answerAutomated PCR machine Quantitative PCR - answerQuantifies the initial amount of DNA RT-PCR - answerCan...

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  • October 18, 2024
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  • 2024/2025
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©SIRJOEL EXAM SOLUTIONS
10/3/2024 9:54 PM


Brock Biology of Microorganisms Chapter 12
Exam Study Guide.



PCR (Polymerase Chain Reaction) - answer✔Multiplying segments of DNA up to a billionfold
during amplification

Thermocycler - answer✔Automated PCR machine

Quantitative PCR - answer✔Quantifies the initial amount of DNA

RT-PCR - answer✔Can make DNA from an RNA template; uses RT to convert RNA into cDNA

Gel Electrophoresis - answer✔Uses Agarose Gel
NAs (negatively charged phosphate groups) migrate through gel toward positive charge

Size Difference in Electrophoresis - answer✔Smaller molecules move faster

NA Hybridization - answer✔Complementary base pairings of single strands of DNA or RNA; 2
different sources to give a hybrid double helix

2 Sources for NA Hybridization - answer✔Southern Blot: Hybridization where DNA is the target
and DNA or RNA is the probe
Northern Blot: Hybridization where RNA is the target and DNA or RNA is the probe

NA Probes - answer✔Segments of ssDNA used in hybridization

FISH (Fluorescent in Situ Hybridization) - answer✔Uses fluorescent probes to target specific
sequences; Can identify pathogens in clinical samples/bacteria in environmental samples

Recombinant DNA - answer✔DNA that has been formed artificially by combining constituents
from different organisms

Molecular Cloning - answer✔Movement of a gene from original source to small and
manipulable genetic element (vector)

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