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Bio 181 Lab Final Exam Questions and Answers

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Bio 181 Lab Final Exam Questions and Answers What is a catalyst? - ANSWER-A substance that increases the rate of a chemical reaction without being used up What is an enzyme? - ANSWER-A biological molecule that increases the rate of a chemical reaction without being consumed, usually a protein....

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  • October 30, 2024
  • 16
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Bio 181
  • Bio 181
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©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED

FIRST PUBLISH OCTOBER 2024




Bio 181 Lab Final Exam Questions and

Answers


What is a catalyst? - ANSWER✔✔-A substance that increases the rate of a chemical reaction without

being used up


What is an enzyme? - ANSWER✔✔-A biological molecule that increases the rate of a chemical reaction

without being consumed, usually a protein.


What is the activation energy? - ANSWER✔✔-The energy required to convert a substrate to a product


How do enzymes catalyze reactions? - ANSWER✔✔-By forming a substrate-enzyme complex. The

enzyme brings the substrates together and put stress to break the bonds. Enzymes create a micro-

environment or break bonds


What are 7 properties of enzymes? - ANSWER✔✔-Most are proteins.




Highly specific to - usually - one substrate.




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, ©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED

FIRST PUBLISH OCTOBER 2024




They form an enzyme-substrate complex:




E + S ↔ ES → P + E




They do not affect the direction of a reaction.




They lower the energy of activation of a reaction.




They are not consumed in the reaction.




They are often highly regulated.


How does an inhibitor work? - ANSWER✔✔-Prevents binding of the target molecule of the enzyme by

binding to the active site or another site


What are the 2 types of inhibitors? - ANSWER✔✔-Competitive and Noncompetitive




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, ©EMILLECT 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED

FIRST PUBLISH OCTOBER 2024




What affects enzymatic activity? - ANSWER✔✔-Substrate specificity, substrate/enzyme concentration,

pH and temperature


How does substrate specificity affect enzymatic activity? - ANSWER✔✔-Substrate specificity is a term for

the fact that each enzyme generally converts only one specific substrate for one specific reaction. The

more specific a substrate is, the enzyme will want to react with that substrate


How does substrate/enzyme concentration affect enzymatic activity? - ANSWER✔✔-If there is too much

substrate compared to the enzyme, enzymatic activity will plateau


Increasing the amount of enzymes will increase the rate of enzymatic activity


How does pH affect enzymatic activity? - ANSWER✔✔-Enzymes function in a certain pH, if the pH

changes more acidic or basic the enzyme's functional groups change. This changes the structure.

Extreme pH can denature enzymes by breaking the hydrogen bonds between them


How does temperature affect enzymatic activity? - ANSWER✔✔-Enzymes function in an optimal

temperature, if the temperature changes their function changes. Extreme high temperatures or cold

temperatures denature an enzymes. Some cold temperatures the enzyme can renature


How are enzymes regulated in the body? - ANSWER✔✔-Optimal temp: 35-40


Optimal pH: 6-8


Feedback inhibition regulates what enzymes are in use and if they are needed


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