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Portage Microbiology Module 4 Exam Questions and Correct Answers Latest Update

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Portage Microbiology Module 4 Exam Questions and Correct Answers Latest Update growth media - Answers nutrient-rich environment for isolating and culturing microorganisms - liquid or solid LB media (lysogeny broth) - Answers Most common nutrient Broth Known for ability to grow vast array of mic...

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  • November 5, 2024
  • 5
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • portage microbiology module 4 exam questions and

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  • Portage Microbiology Module 4
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Portage Microbiology Module 4 Exam Questions and Correct Answers Latest Update 2024-2025

growth media - Answers nutrient-rich environment for isolating and culturing microorganisms - liquid or
solid

LB media (lysogeny broth) - Answers Most common nutrient Broth

Known for ability to grow vast array of microbes

Selective Media - Answers allows for growth of specific microbes, while restricting the growth of other
microbes




often used to grow human pathogens, or slower growing microbes. The selectivity allows for discounting
foreign unwanted microbes

Differential Media - Answers allows growth of several types of microbes and displays visible differences
among those microbes

ex. both gram(-) ecoli v salmonella as ecoli ferments lactose and growth w lactose shows red, where
salmonella remains white/tan

Enriched Media - Answers used to grow fastidious orgs - complex growth requirements that will cease to
grow if not exact.

Agar - Answers Polysaccharide derived from seaweed extract

Solid & smooth allowing growth of colonies in isolated dots

Covering entire plate = lawn

more agar = more firm/

LB agar - Answers Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of
bacteria; LBagar capable of wide variety of micro'o's.

LB is non-selective and non-differential.

Trypticase Soy Agar - Answers TSA or TSAYE - identical in color to LBagar

Multipurpose media w high variety

Non-selective & non-differential

Blood Agar - Answers BAPlates, derivative of TSA combined with mammalian blood, at [5-10%]

, BAP are red

BAP rich, non-selective & are differential

RBC allow for growth of fastidious micro'o's IE strep

Allows for detection of HEMOLYTIC ACTIVITY

BAP Hemolysis - Answers Alpha h- greenish-browish color, due to incomplete/partial lysis of RBC

Beta h- capacity a microbe has to completely lyse RBC

Gamma h- lack of hemolytic activity, resulting colonies often white/tan growing on red background of
agar plate

Columbia CNA agar - Answers Red color, enriched selective and differential

Used for isolation of gram(+) bact



Media suppresses growth of Gram(-) bact = contains antimicrobials colistin and nalidixic acid

Chocolate Agar - Answers CHOC, or CBA Chocolate Blood Agar

enriched, non-selective, non-differential derivative of BAP



Plate contains RBC that have been lysed via heat PRIOR to plating (differs BAP as those are lysed on the
plate)



The cooking of RBC gives dark choc color



Lysed red cells release various growth factors into media required for cultivation of fastidious,
pathogenic bact.



Enriched

examples: Haemophilus influenzae and Neisseria Meningitidis -- gram (-)

MacConkey Agar (MAC) - Answers -*Selective* for *gram-negative bacteria*. Inhibits the grow of gram-
positive bacteria and fungi w crystal violet and bile salts

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