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Microbio 150 Lab exam 1 PVCC Questions & Answers Already Graded A+
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Why is desiccating your inoculating loop important? When should this be done? - ️️To ensure it is sterilized, done so no cross contamination happens Bacteria in the Mycobacterium genus have the presence of what component in the cell wall that causes them to be acid-fast? - ️️Peptidoglyca...

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  • November 12, 2024
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Microbio 150 Lab exam 1 PVCC
Why is desiccating your inoculating loop important? When should this be done? -
✔️✔️To ensure it is sterilized, done so no cross contamination happens

Bacteria in the Mycobacterium genus have the presence of what component in the cell
wall that causes them to be acid-fast? - ✔️✔️Peptidoglycan

Where should used gloves be disposed of in the microbiology lab - ✔️✔️In the bio-
hazard waste container

Before putting a dust cover over the microscope and placing it in the cabinet, which
objective lens should be rotated over the stage - ✔️✔️4x

On the microscope, what concentrates the light on the specimen? - ✔️✔️Condenser
lens

On the microscope, what bends light passing through the specimen to create a real
image - ✔️✔️Objective lens

On the microscope, what allows for more or less light to be directed onto the specimen -
✔️✔️Iris diaphragm

When preparing to view a specimen on a compound microscope in the lab, one should
__________ begin with the 4x objective - ✔️✔️Always


On the microscope, what gives you clarity of the image - ✔️✔️Resolution


During an aseptic transfer, when do you pass the mouth of the test tube through the
flame? - ✔️✔️Before and after dipping loop in

During the broth to slide aseptic transfer, a __________ was used to obtain a pure
culture from a __________. - ✔️✔️Loop, broth

Prior to staining, smears of microorganisms are heat-fixed in order to - ✔️✔️Attach it
firmly to the slide

The term that refers to the purposeful addition of microorganisms into a nutrient medium
is - ✔️✔️Inoculation

, True or false: In the microbiology lab, it is acceptable to move your loop around while
waiting for it to dry. - ✔️✔️False

Which stain was used during the negative staining procedure? - ✔️✔️Nigrosin

Which stain was used during the simple staining procedure? - ✔️✔️Methylene blue

True or false: During a negative stain, the bacteria are heat fixed. - ✔️✔️False

After the decolorizer has been added, gram-positive organisms appear __________
and gram-negative organisms appear __________. - ✔️✔️Purple; colorless

Why is iodine (or a mordant) used in the Gram staining process? - ✔️✔️It forms a
complex with crystal violet

True or false: bacterial slides are not heat-fixed prior to beginning to Gram staining. -
✔️✔️False

When completing a bacterial transfer from a broth, where should the cap be located
while you are inserting your inoculating instrument in the bacterial growth? - ✔️✔️The
cap should be held in the pinky of your dominant hand

- ✔️✔️Agar slant

- ✔️✔️Broth tube

- ✔️✔️Agar plate

solidified in an upright angle - ✔️✔️Agar deep

What is the purpose of a simple stain? - ✔️✔️To quickly visualize microorganisms
because they are virtually transparent.

True or false: Basic stains work because the positively charged chromophore repels the
positively charged bacterial cell. - ✔️✔️False

What is the colored, charged region of a stain called? - ✔️✔️The chromophore

- ✔️✔️Negative stain

Positive or negative - ✔️✔️Negative gram stain

Positive or negative - ✔️✔️Positive gram stain

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