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Bio 311C- Sata Final Exam Prep | Questions, all Solved 100% Correct with Verified Answers
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Bio 311C- Sata Final Exam Prep | Questions, allSolved 100% Correct with Verified Answers
nucleolus - ✔✔organizing center in the nucleus that makes ribosomes from ribosomal rna
nuclear pores - ✔✔pores on the nuclear membrane allow chemicls and mRNA to pass
nuclear lamina - ✔✔protein membrane on inside of nucleus made of protein laminarin
nuclear proteins - ✔✔dna polymerases (for replication), dna binding proteins, etc. stored in
the nucleus
nucleoplasm - ✔✔fluid inside a nucleus, included suspended DNA, RNA, proteins, fibers,
nuleotides
ribosomes - ✔✔in cytoplasm, synthesize proteins from DNA
cisternae - ✔✔space in the ER
smooth ER - ✔✔no ribosomes, synthesizes lipids, catalyzes glycogen to glucose, detox
rough ER - ✔✔has ribosomes attached, makes proteins, makes cell membranes, makes
membrane-bound proteins
restriction endonuclease - ✔✔recognizes and cuts specific sequences of DNA for the
insertion of new (foreign) DNA
DNA Ligase - ✔✔connects 2 DNA fragments through phosphodiester bond
, DNA/RNA Polymerase - ✔✔connect opposite base pairs during replication
reverse transcriptase - ✔✔turns mRNA into cDNA
vectors - ✔✔autonomously replicating DNA molecules that carry foreign DNA to insert into a
host cell to replicate
plasmid vector - ✔✔(bacteria) uses lac-z to swap DNA
phage vector - ✔✔(viral) includes a left arm and a right arm
hybridization - ✔✔technique of analyzing DNA, uses north (Rna), south (Dna) or west
(proteins)
PCR - ✔✔polymerase chain reaction, multiplies DNA through denaturation, annealing
primers, and extention (uses a thermostable Dna polymerase)
microarray - ✔✔(method of analyzing dna) detect rna of 100s of genes at once
main technique of cloning - ✔✔transforming a bacteria, plant or animal cell :in bacteria,
must remove introns and optimize codon use (since codons for bacteria are different than
codons for an animal)
trp operon - ✔✔usually on, when trp accumulates over a certain level the trp operon is
surpressed
lac operon - ✔✔basically converts lactose to glucose - active when theres a lot of lactose
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