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BIOL 200 Mcgill Questions And Answers With Latest Solutions
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BIOL 200 Mcgill Questions And Answers With Latest Solutions RNA polymerase ANS Core enzyme + sigma factor = Holoenzyme Sigma Factor ANS Finds promoter (motif rich in T/A) Core Enzyme ANS Two alpha and two beta proteins Holoenzyme ANS Scans DNA to find promoter (help of Sigma factor) f...

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  • November 21, 2024
  • 11
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • biol 200 mcgill
  • biol 200 mcgill questions and answers with latest
  • biol 200 mcgill questions and answers
  • rna polymerase ans core enzyme sigma factor

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BIOL 200 Mcgill Questions And Answers With Latest
Solutions
RNA polymerase ANS Core enzyme + sigma factor = Holoenzyme



Sigma Factor ANS Finds promoter (motif rich in T/A)



Core Enzyme ANS Two alpha and two beta proteins



Holoenzyme ANS Scans DNA to find promoter (help of Sigma factor) forms closed complex.



Ribosome ANS RNA + Protein. Large subunit 50s, small is 30.
Together = 70s



Initiation Factors (3) ANS IF1/IF3 recruits 30s ribosome,
IF1/IF2 recruits 50s subunit, forming the 70s



Elongation Factors (Efs) ANS to make peptides bonds between aa, ribozyme 23s rRNA makes
reaction


Release factors (RFs) ANS RF1/RF2 mimics a tRNA.
RF3 allows for disassembly of 70s ribosome, release of polypeptide.



DNA Helicase ANS Seperates the DNA strands



Tropoisomerase ANS relieves supercoils when unwinding the DNA



Primase ANS RNA polymerase, adds primer

, Pol alpha ANS DNA polymerase extends the primer with dNTPs for 20 bp (approx)



pol E/Rfc/PCNA complex ANS For leading strand



pol epsilon (pol e) ANS DNA polymerase which extends the leading strand with dNTPs.



Rfc (Replication factor C) ANS loads/unloads complex on strand.



RPA (Replication protein A) ANS Keeps the DNA template in optimal conformation for
transcription. (Template could fold otherwise).



PCNA ANS Keeps the DNA polymerase onto the template during transcription. It is a
homotrimeric protein (3 identical subunits)



pol d/Rfc/PCNA complex ANS Extends the lagging strand with dNTPs.
Replaces the pol alpha/primase complex


pol delta (pol d) ANS DNA polymerase which extends the lagging strand with dNTPs.



Ribonuclease H and FEN-1 ANS Removes the RNA from the primer, pol d replaces with
complementary base pairs.



DNA Ligase ANS Seals the backbone break.



Large T-antigen ANS Hexamer (6 subunits).
type of helicase, encoded by the viral genome.
Allows for very fast replication.



Aminoacyl-tRNA synthetase ANS Links amino acid to its corresponding tRNA.

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