Lactose fermenters - Study guides, Class notes & Summaries

Bile Esculin Agar correct answers a selective and differential medium which is used to presumptively identify Enterococcus and group D Streptococcus based on the ability of an organism to hydrolyze esculin BEI Agar positive correct answers Black Precipitate forming around bacteria on plate BEI negative correct answers no precipitate Mannitol Salt Agar correct answers selective (sodium chloride) and differential (mannitol) Staphylococci thrive and grow on the agar S. Aureus ferments ma...

Growth media - nutrient-rich environment for isolating and culturing microorganisms, includes sugars, amino acids, and vitamins, also called nutrient broth, grows microbes in a suspension Lysogeny broth - most common nutrient broth, can grow a vast array of microbes, plates are yellow, non-selective and non-differential media, commonly used for growing e coli (easily produces recombinant proteins) Selective media - suppress growth of unwanted bacteria and encourage growth of desired microb...

BI 234 LAB PRACTICAL EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED BY EXPERT Gram negative bacteria Thin layer of peptidoglycan. Contains outer membrane (lipo-polysaccharides, lipoproteins, phospholipids) with porin. gram positive bacteria Bacteria that have a thick peptido glycan cell wall, and no outer membrane. They stain very darkly (purple) in Gram stain. endospore stain A differential stain used to detect the presence and location of spores in bacterial cells. TSA plat...

TSI correct answer: Triple Sugar Iron Yellow= acid production or fermentation of the sugars in the slant or butt. Gas production= indicated by the agar lifting up or breaking Red= negative or uninoculated Black= H2S production due to FeS precipitation (think salmonella or proteus) The source of Sulfur for H2S production by bacteria is the amino acid Cysteine TSI additional correct answer: Triple Sugar-Iron agar slants. is a differential medium containing 3 sugars called glucose...

FeCl3 Used in a PAA agar to detect presence of phenylpyruvic acid positive green color. MAC agar What stain do you do on Day 1 of the unknown lab? You may Gram stain from your unknown broth to confirm if the bacteria are Gram-positive or Gram-negative. A throat culture performed by using a throat swab is used to detect which organism that causes strep throat? Group A Streptococcus Bacteria Which staphylococci is the most important bacteria in human disease? Staphylococcus Aureus W...

4 stages in a bacterial growth are: - Lag phase, Exponential (Log), Stationary, Death Acid-fast staining - (differential staining) Add carbol fuchsin (primary stain) then heat over flame for few minutes. 2.Wash and then add a decolorizer (5% HCl and 95% ethyl alcohol) to remove primary color from nonacid-fast organisms. Acid-fast bacteria will retain the primary color. Alexander Fleming - discovered penicillin (the first antibiotic discovered!) Anton Van Leeuwenhoek - was the first to obser...

Portage Learning BIOD 171 Microbiology Lecture Exam Key 1-6 Exams 1. What are the two major types of nucleic acids and their roles? DNA- deoxyribonucleic acid RNA- Ribonucleic acid DNA- Cellular life has DNA and a role that DNA has is long-term storage of information, it holds the characteristics of living organisms. RNA- Is the carrier of information in form of amino acid sequence. It converts the information in the DNA and synthesizes proteins Tru...

Why is differential media typically inoculated with isolated colonies that have been previously cultured on general growth media? The general growth media used for the isolation of microorganisms from unknown clinical or non-clinical samples is referred to as differential media because the presence of indicators within the culture media allows for the growth of bacteria with distinct characteristics. 3. Use a textbook or a reputable internet source (such as ) to research and describe a sce...

What does TSI stand for? correct answer: Triple Sugar Iron How do you inoculate a TSI? correct answer: Stab and Drag What does a TSI slant agar select for? correct answer: Differentiates between non-fermenters, glucose fermenters and fermenters of lactose and/or sucrose. It is used to identify intestinal bacteria. What does a black medium on SIM agar indicate? correct answer: Positive for sulfur reduction and H₂S production What agent do you add to the indole test and what orga...

BIO 250L V2 Lab 4 (Selective Media & Agar) Questions With 100% Correct Answers | Latest Update Graded A+. “Pre-Lab Questions” 1. What is the difference between chemically defined and chemically complex media? Give either a clinical or environmental research example for which each media type would be the most appropriate choice for culturing microorganisms. Chemically defined media have known chemical compositions, whereas complex media have unknown chemical compositions. Chemically defi...