Summary

Summary Edexcel A-Level Unit 3 Revision Notes (WBI13) [New spec]

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PEARSON (PEARSON)

It includes all practicals for International AS Level specifications. Easy to read, understand and study. I used these notes for Unit 3 and got an overall A in my AS level Biology. And I got an A* for my AL predicted grade. Summarise these notes by adding the common answers in the mark schemes!...

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Uploaded on June 20, 2022
File latest updated on June 20, 2022
Number of pages 12
Written in 2021/2022
Type Summary

ial
biology
core practicals
edexcel
advanced level
unit 3
british
british curriculum
new spec
al
pearson
notes
international advanced level

Benedict’s reagent for reducing sugar / biuret test
Dependent Colour change of Benedict’s reagent

Independent Concentration of reducing sugar

Control - Temperature (thermostatically control water bath)
- Volume of Benedict’s solution
- Time
- Volume of solution being tested

Risk - Liquid (spillage)
- Glassware: cuts from sharp objects

Colour change Biuret test: blue to purple

Benedict’s reagent

Procedure 1. 5 different protein/ glucose concentration + examples
2. Same volume of protein/ glucose solution
3. Same volume of the biuret / benedict solution
4. Swirl the mixture of the same time
5. Thermostatically controlled water bath → keep the
temperature the same
6. Standardize the cuvette
7. Colourimeter to absorbance
8. Draw a table and record the data
9. Plot the graph absorbance against protein concentration

, Structure of mammalian heart by dissection -daphnia (reference)

Dependent beats per minute’ for heart rate

Independent Pesticide used

- allow Daphnia to acclimatize / equilibrate in the pesticide
- pencil and dots for set time period (30s) , count heart beats for
stated time
- controlling temperature
- obtaining Daphnia from pesticide-free water
- use of a cavity slide/ immobilizing Daphnia with cotton wool
- same concentration of pesticides
- same size Daphnia
- take several readings and calculate a mean

Why pesticide - pesticides may be water soluble ;
enter Daphnia - so enter by diffusion / facilitated diffusion
and cause a - absorbed by heart muscle cells / alters nerve function / alters
change in (muscle) contraction
heartbeat

Error - Daphnia not be same size
- Not enough data
- Too high conc kill the pesticide
- Counting of the heart beat not accurate

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