194 marks
Q1.
The diagram shows the structure of two α-glucose molecules.
(a) On the diagram, draw a box around one chemical group in each glucose molecule
used to form a glycosidic bond.
(1)
(b) A precipitate is produced in a positive result for reducing sugar in a Benedict’s test.
A precipitate is solid matter suspended in solution.
A student carried out the Benedict’s test. Suggest a method, other than using a
colorimeter that this student could use to measure the quantity of reducing sugar in
a solution.
,In an investigation, a student wanted to identify the solutions in two beakers, A and B.
She knew one beaker contained maltose solution and the other beaker contained glucose
solution. Both solutions had the same concentration.
She did two separate biochemical tests on a sample from each beaker.
Test 1 – used Benedict’s solution to test for reducing sugar.
Test 2 – added the enzyme maltase, heated the mixture at 30 °C for 5 minutes, and then
Used Benedict’s solution to test for reducing sugar.
Maltose is hydrolysed by maltase.
The student’s results are shown in the table below.
Colour of solution after testing
with Benedict’s solution
Beaker Test 1 Test 2
A red red
B red dark red
(c) Explain the results for beakers A and B in the table.
Beaker A __________________________________________________________
In Test 1, the student used a measuring cylinder to measure 15 cm3 of solution from a
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, beaker. The measuring cylinder gives a volume with an uncertainty of ±1 cm3. She used a
graduated syringe to measure 5.0 cm3 of Benedict’s solution. The graduated syringe gives
a volume with an uncertainty of ± 0.5 cm3. She mixed these volumes of liquid to do the
biochemical test.
(e) Calculate the percentage error for the measurements used to obtain a 20 cm3
mixture of the solution from the beaker and Benedict’s solution. Show your working.
(2)
(c) Following digestion and absorption of food, the undigested remains are processed
to form faeces in the parts of the intestine below the ileum.
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, The faeces of people with constipation are dry and hard. Constipation can be
treated by drinking lactulose. Lactulose is soluble, but is not digested or absorbed in
the human intestine.
Use your knowledge of water potential to suggest why lactulose can be used to help
people suffering from constipation.
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