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Summary OCR Biology A level 6.2.1 Cloning and Biotechnology £3.69
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Summary OCR Biology A level 6.2.1 Cloning and Biotechnology

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Summary notes for topic 6.2.1 Cloning and biotechnology of OCR Biology A level Module 6. Detailed electronic notes with diagrams.

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  • June 22, 2023
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PCR uses less space
 DNA + enzymes more compact than whole cells
 No growth medium required
 In vivo requires many plates to be stored/incubated/refrigerated
PCR safer
 Uses DNA & enzymes
 Does not use whole cells which could cause contamination
PCR can use lower quality DNA
 Can use old prehistoric/forensic DNA
PCR less labour intensive/easier
 Set to run and left
 Gene identified & cloned in one stage
 In vivo requires more purification at end
In vivo less prone to mutation
 Taq polymerase occasionally inserts wrong base
 Early mutation reproduced many times in PCR
 Exact correct sequence needed for making therapeutic proteins
In vivo less expensive
 PCR primers/Taq polymerase/high temperatures expensive
 Materials for growing bacteria cheap
In vivo less technically complex
 Conditions not so critical
 Optimising PCR takes time
In vivo useful when gene less well known/as longer piece of DNA can be cloned
 Searching for new gene
 Obtains complete gene
o PCR has limited size




6.2.1. Cloning and Biotechnology
Biotechnology is the industrial use of living organisms (or parts of living organisms) to produce food, drugs or useful
enzymes

Microorganisms are often used in biotechnological processes;
 Fast growth
 Large amount of product
 Can be genetically engineered
o Avoids problem with side effects/allergic effects
 Process can occur at low temperatures/pressures
o Low temperatures cheaper/safer to maintain
 Products are pure so are easier to separate
 Can grow on unwanted food
 Ethical advanages


Rate of growth of population slows:
 One or more of nutrients are
beginning to run out
 Birth rate =Death rate




Nutrients run out
Organisms adjust to  Increased levels of
surrounding conditions. toxic waste products
 Taking in water,  Death rate > Birth rate
cell expansion,

, Number of bacteria increase rapidly.
 Cells growing/dividing at their maximum rate
for the particular conditions they are in
 Length depends on how quickly organisms:
o Reproduce
o Take up nutrients




How can enzymes be immobilised;

Adsorbtion:
 Bound to porous clay/carbon/resin/glass
 Hydrophobic interactions/ionic links
o Not very strong, enzymes becomes detached - LEAKAGE
o Can give high reaction rates



Covalent Bonding:
 Enzymes covalently bonded
 Cross linking enzymes to each other and to clay
 (using glutaraldehyde)
o Does not immobilise large quantity of enzyme
o Binding very strong - LOW LEAKAGE


Entrapment:
 Enzymes trapped in alginate beads/cellulose network
 Trapped in NATURAL STATE (not bound to another molecule/active site
unaffected)
o Reaction rate reduced
o Substrate molecules need to get through trapping barrier


Membrane separation:
 Enzymes and substrate either side of partially
permeable membrane
 Enzyme solution on one side of membrane, substrate
solution passed on other side
o Substrate molecules small enough to pass
through membrane so reaction can take place
o Product molecules small enough to pass back
through membrane




Method depends on:
 Ease of preparation
 Cost

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