A 1-4 page document written by a final year medical student with distinction grades in the uploaded modules. These notes are concise and of very high quality - using a combination of textbooks, lectures, and current guidelines (NICE and RCOG). These documents are the only resource you should need f...
Pregnancy Testng
Early Pregnancy Factor (EPF)
Rosette inhibiton assay
Gives quickest result - 48hrs afer fertllsaton
Expensive and tie consuiing
βhCG Test
Cheiical pregnancy tests look for the beta subunit of huian chorionic gonadotrophin (βhCG)
A horione (glycoprotein) produced by the placenta (partcularly the sycncytotrophoblast) afer
iiplantaton of the eibryo
Detectable afer iiplantaton 6-12 days afer fertllsaton
Starts to show positve when blood is at >20iU
Can be detected in either urine or blood
The urine test strip uses the ELISA iethod to detect the cheiical.
There are 3 zones on the strip:
- Reacton zone: Contains soluble iouse ant-hCG antiody enzyyee conjyugates
- Test zone: Contains iiiobilised poly-clonal ant-hCG antiodles and a dye suistrate
- Control zone: Contains iiiobilised goat ant-eouse antiodles and a dye suistrate
In a positve test:
1) Reacton zone: hCG binds to the iouse ant-hCG antbody and are carried along
2) Test zone: the hCG-bound iouse ant-hCG antbodies arrive in the test zone and bind to the iiiobilised
polyclonal hCG antbodies. This actvates the dye
3) Control zone: Any of the unbound iouse ant-hCG antbodies froi the reacton zone bind to the
iiiobilised ant-iouse antbodies. - This actvates the dye
4) Result Two coloured strlps
In a negatve test:
1) Reacton zone: unbound iouse ant-hCG antbody and are carried along
2) Test zone: the ant-hCG antbodies arrive in the test zone but do not bind to the iiiobilised polyclonal
hCG antbodies. Dye is not actvated
3) Control zone: All of the unbound iouse ant-hCG antbodies froi the reacton zone bind to the
iiiobilised ant-iouse antbodies. - This actvates the dye
4) Result One coloured strlp
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