Summary
Summary BHCS2005 Clinical Haematology and Biochemistry - Methods in Haematology
- Institution
- Plymouth University (UOP)
An overview of the methods in haematology aspect of the BHCS2005 module at the university of Plymouth.
[Show more]
Content preview
Methods In HaematologyFull Blood Count
Automated analysers detect
o Number and size of RBCs, WBCs and platelets
o Haemoglobin concentration
o Haematocrit
Values
Red cell count
o Male - 4.5-6.5 x 10^12/L
o Female - 3.9-5.6 x 10^12/L
Haemoglobin
o Male - 13.5-18 g/dL
o Female - 11.5-16 g/dL
White cell count
Haematocrit
Haematocrit is the packed cell volume
o 40-52% in males
o 38-48% in females
Mean corpuscular haemoglobin
o The amount of oxygen carrying haemoglobin inside RBCs
Mean corpuscular haemoglobin concentration
o Concentration of haemoglobin per RBC
Red cell distribution width
o Variation in size of RBCs
Inflammatory Tests
Erythrocyte Sedimentation Rate
, o Non-specific test for inflammation
o Increased fibrinogen with inflammation makes RBCs sticky and
sediment faster
o Inexpensive, quick and simple
o Affected by a variety of factors
C-reactive protein
o Acute inflammatory response
o Most rapid response
o Wide reference
o Expensive
Blood Smear
Performed manually to detect size, number and type of cells
Additional test to ESR
Giemsa stain used to detect morphology of granulocytes and platelets
Automated Analysers
Spectrophotometry
o Measures haemoglobin concentration by converting to
cyanmethemoglobin and measuring absorbance at 540nm
Impedance
o Measures cell size and number for RBC, WBC, platelets and mean
cell volume
Flow cytometric analysers
o Detect cell shape, size, granularity and epitopes
o Individual cells are passed through a laser beam
o Forward scatter for size, side scatter for complexity
o Automatic count cells within known regions
Flow cytometry gating
o One parameter
Cell count and measurement of fluorescence
o Two parameter
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller hawkinsella2002. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £8.66. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77254 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy revision notes and other study material for 14 years now