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applied science - chemistry - unit 19 C part 4 section 2 - distinction level £7.16
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applied science - chemistry - unit 19 C part 4 section 2 - distinction level

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Essay of 8 pages for the course Unit 19 - Practical Chemical Analysis at PEARSON (HPLC Chromatograms)

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  • May 14, 2024
  • 8
  • 2023/2024
  • Essay
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By: cliodhnafagan97 • 7 months ago

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By: cliodhnafagan97 • 7 months ago

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chiam
HPLC Chromatograms

I will be analysing different solvents used to extract a sample of physica semipinnata (rosette lichen)
against a reference.

Explain how to calculate peak area. Include an example calculation.



To calculate the peak are you must measure the heigh of the peak and multiply it by the half of the
width. it cam be represented as;

Height of peak/ hath of the width of peak

Example

Height is 15

Width is 4

The it will be

7 /2 = 3.5

3.5 is the peak area

, Reference Chromatogram.




Reference compounds



Peak number Standards for Compounds present in physcia
semipinnata

1 lecanoric acid

2 methyl-β-orcinol carboxylate

3 ethyl haematommate

4 evernic acid

5 obtusic acid

6 atranorin

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