BIO 245 LAB FINAL EXAM
LAB SAFETY - ANS-1. Know location of exits, fire extinguisher, eyewash station & first
aid kit.
2. NEVER!!! Eat, drink, apply cosmetics... in the laboratory.
• KEEP HANDS AWAY FROM MOUTH, EYES, NOSE.
3. Inform instructor if pregnant or immunocompromised or any other special medical
conditions
4. DRESS the Part: goggles, gloves, lab coat, closed-toe shoes, tie back long hair.
5. Existing cuts should be bandaged.
6. NEVER remove anything from the lab (ie. cultures, chemicals, slides, etc)
7. Always thoroughly wash your hands before you leave the lab.
8. AVOID spills - center materials, stay organized. DO NOT TIP TEST TUBES.
9. Treat all live cultures as POTENTIAL PATHOGENS.
10. Never pipette fluids by mouth.
11. PROPERLY LABEL test tubes and Petri dishes.
12. Report all accidents and spills.
13. Know the disposal areas.
• 14. ASK when you do not know.
• 15. Test tubes are ALWAYS kept in a test tube rack. This includes transporting a test
tube from one location to another.
• 16. DO NOT place contaminated instruments on the bench top - sterilized first with
burner.
• 17. Windows and doors are kept closed.
• 18. NEVER use hands to pick up broken glass.
• 19. Turn off gas burner when NOT in use. Never leave flame unattended. Constantly
be aware of your burner.
The Compound Light Microscope - ANS-Enhances range of human senses; allows for
detailed observation
Basic Parts of the Compound Light Microscope - ANS-
Revolving Nosepiece - ANS-Rotates to change magnification
Arm & Base - ANS-Supporting piece
Stage - ANS-Platform to place specimen
, Ocular lens - ANS-Eyepiece (10x)
Objective Lens - ANS-4x, 10x, 40x, and 100x
Diaphragm - ANS-Regulates amt of light
Coarse Adjustment Knob - ANS-*First Focusing*
with 4x and 10x lenses only
Fine Adjustment Knob - ANS-*Sharpen Focus*
under 4x, 10x, 40x, and 100x
Use of the Compound Light Microscope - ANS-To focus: Raise stage to HIGHEST
position and focus with COARSE ADJ. KNOB AWAY from specimen.
- Use coarse adjustment focusing knob with 4x 10x objective ONLY!!
NEVER USE COARSE ADJUSTMENT KNOB with HIGH POWER (40X) LENS!!!
Magnification - ANS-The apparent increase in the size of an organism
Total Magnification - ANS-
Working Distance - ANS-The distance between the slide and the bottom of the objective
lens
Field of View - ANS-How much of the slide can be seen when looking through the
ocular lens
Resolution - ANS-Ability to see two items as separate and discrete
(CLARITY of an image)
Resolving Power - ANS-Based on the quality of the lens and the properties of the
energy source
Aseptic Technique - ANS-Any techniques employed to avoid contamination.
- All inoculating instrument (loops and needles) should be sterilized prior to use by
flaming
Ubiquity - ANS-Appearing everywhere
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