100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
Bio 245 Lab Final Dr. V JMU Questions with 100% Actual correct answers | verified | latest update | Graded A+ | Already Passed | Complete Solution £6.54
Add to cart
Quickly navigate to
Preview
  2.  
  3. BIO 245
  4.  
  5. BIO 245
  6.  
  7. BIO 245

Exam (elaborations)

Bio 245 Lab Final Dr. V JMU Questions with 100% Actual correct answers | verified | latest update | Graded A+ | Already Passed | Complete Solution

 0 purchase

Bio 245 Lab Final Dr. V JMU Questions with 100% Actual correct answers | verified | latest update | Graded A+ | Already Passed | Complete Solution

Preview 2 out of 11  pages

Document information

  • June 19, 2024
  • 11
  • 2023/2024
  • Exam (elaborations)
  • Questions & answers

Subjects

  • bio 245 lab final dr v jmu questions with 100 a

Written for

All documents for this subject (198)

Seller

avatar-seller
DoctorHkane

Reviews received

Content preview

Bio 245 Lab Final Dr. V JMU
describe gram negative bacteria - ANS-Thin cell walls; pink after gram stain (b/c the thin
cell walls cannot retain the crystal violet stain, and retain the safranin stain instead)

describe gram positive bacteria - ANS-Thick cell walls; purple after gram stain (b/c the
thick cell walls retain the crystal violet stain)

advantages of wet mounts - ANS-Cells are still living, organelles are still in tact, can
observe processes of life such as cell division or feeding, motility can be observed

advantages of stains - ANS-Allows differentiation between some cells, cells aren't
moving,

name the three types of stains - ANS-gram, simple, and negative stains

describe a gram stain - ANS--differential; tells if cell is gram positive or gram negative
-Pink = gram negative
-Purple = gram positive
-Crystal violet (stains organisms purple)
-Iodine (mordant, keeps stain in better)
-Alcohol wash (washes out stain in gram negative bacteria)
-Safranin (pink dye; makes gram negative pink, but doesn't make gram positive
pink/they stay purple)
--Chris Is Always Sassy = Crystal violet, Iodine, Alcohol wash, Safranin

describe a simple stain - ANS-"simply" tells presence of cells, arrangement, size, and
morphology

describe a negative stain - ANS-stains the background rather than the actual organism;
NO heat fixing

describe the three mirphologies - ANS-bacillus: rod shaped
coccus: circular shaped
spiral: spiral shaped

name the two types of media - ANS-solid and liquid media

what are the two types of solid media - ANS-plates and slants

, what is a solid plate media used for - ANS-short term storage, provide more surface
area, for everyday work

what is a solid slant media used for - ANS-long term storage

what is a liquid broth media used for - ANS-for some organisms that grow better in broth
or liquid, good for fermentation studies and for biochemical tests

what is TSA media and what is it used for - ANS-Tryptic soy agar
undefined/complex media. Solid agar containing growth media required for organisms.
Non-selective, non differential

what is TSB media and what is it used for - ANS-Tryptic Soy broth
TSA but in broth form, used for organisms that grow better in liquid

what is TSY media and what is it used for - ANS-Tryptic Soy Yeast Agar
same as TSA, but yeast extract is added

what was FTB media and what is it used for - ANS-fluid thioglycollate broth
-used to test aerotolerance
-contains sodium thioglycollate and L-cystine which reduces oxygen to water.
-contains resazurin which is an indicator of oxygen (pink when oxidized/oxygen present;
clear when reduced/oxygen absent), and agar which slows diffusion of oxygen
-contains a small amount of agar which slows the oxygen diffusion through the broth

what is EMB media and what is it used for - ANS-Eosin Methylene Blue
selects for gram negative bacteria, differentiates between lactose fermenting bacteria

what is a lawn culture - ANS-uses the same initial technique as spread plate, but tests
the reaction of pure cultures to various substances (antibiotics) using Mueller-Hinton
Agar Uses Mueller-Hinton agar which tests for antibiotic resistance

what are agar deeps - ANS--solid agar that is devoid of oxygen (after autoclaving). After
about a week there is an oxygen gradient which is useful for testing aerotolerance. Also
useful in culturing bacteria that require less oxygen
-Solid agar that is devoid of oxygen after autoclaving. A week after autoclaving, the
tubes have an oxygen gradient

what is SDA media and what is it used for - ANS-Sabouraud Dextrose Agar

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller DoctorHkane. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for £6.54. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

69052 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy revision notes and other study material for 15 years now

Start selling
£6.54
  • (0)
Add to cart
Added