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Bio 245 Lab Final Dr. V JMU Questions with 100% Actual correct answers | verified | latest update | Graded A+ | Already Passed | Complete Solution £6.27
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Bio 245 Lab Final Dr. V JMU
describe gram negative bacteria - ANS-Thin cell walls; pink after gram stain (b/c the thin
cell walls cannot retain the crystal violet stain, and retain the safranin stain instead)
describe gram positive bacteria - ANS-Thick cell walls; purple after gram stain (b/c the
thick cell walls retain the crystal violet stain)
advantages of wet mounts - ANS-Cells are still living, organelles are still in tact, can
observe processes of life such as cell division or feeding, motility can be observed
advantages of stains - ANS-Allows differentiation between some cells, cells aren't
moving,
name the three types of stains - ANS-gram, simple, and negative stains
describe a gram stain - ANS--differential; tells if cell is gram positive or gram negative
-Pink = gram negative
-Purple = gram positive
-Crystal violet (stains organisms purple)
-Iodine (mordant, keeps stain in better)
-Alcohol wash (washes out stain in gram negative bacteria)
-Safranin (pink dye; makes gram negative pink, but doesn't make gram positive
pink/they stay purple)
--Chris Is Always Sassy = Crystal violet, Iodine, Alcohol wash, Safranin
describe a simple stain - ANS-"simply" tells presence of cells, arrangement, size, and
morphology
describe a negative stain - ANS-stains the background rather than the actual organism;
NO heat fixing
describe the three mirphologies - ANS-bacillus: rod shaped
coccus: circular shaped
spiral: spiral shaped
name the two types of media - ANS-solid and liquid media
what are the two types of solid media - ANS-plates and slants
, what is a solid plate media used for - ANS-short term storage, provide more surface
area, for everyday work
what is a solid slant media used for - ANS-long term storage
what is a liquid broth media used for - ANS-for some organisms that grow better in broth
or liquid, good for fermentation studies and for biochemical tests
what is TSA media and what is it used for - ANS-Tryptic soy agar
undefined/complex media. Solid agar containing growth media required for organisms.
Non-selective, non differential
what is TSB media and what is it used for - ANS-Tryptic Soy broth
TSA but in broth form, used for organisms that grow better in liquid
what is TSY media and what is it used for - ANS-Tryptic Soy Yeast Agar
same as TSA, but yeast extract is added
what was FTB media and what is it used for - ANS-fluid thioglycollate broth
-used to test aerotolerance
-contains sodium thioglycollate and L-cystine which reduces oxygen to water.
-contains resazurin which is an indicator of oxygen (pink when oxidized/oxygen present;
clear when reduced/oxygen absent), and agar which slows diffusion of oxygen
-contains a small amount of agar which slows the oxygen diffusion through the broth
what is EMB media and what is it used for - ANS-Eosin Methylene Blue
selects for gram negative bacteria, differentiates between lactose fermenting bacteria
what is a lawn culture - ANS-uses the same initial technique as spread plate, but tests
the reaction of pure cultures to various substances (antibiotics) using Mueller-Hinton
Agar Uses Mueller-Hinton agar which tests for antibiotic resistance
what are agar deeps - ANS--solid agar that is devoid of oxygen (after autoclaving). After
about a week there is an oxygen gradient which is useful for testing aerotolerance. Also
useful in culturing bacteria that require less oxygen
-Solid agar that is devoid of oxygen after autoclaving. A week after autoclaving, the
tubes have an oxygen gradient
what is SDA media and what is it used for - ANS-Sabouraud Dextrose Agar
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