100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
CP5 write up - Prepare and stain a root tip squash to observe the stages of mitosis £2.99
Add to cart
Quickly navigate to
Preview
  2.  
  3. PEARSON (PEARSON)
  4.  
  5. Biology 2008
  6.  
  7. Core Practicals

Exam (elaborations)

CP5 write up - Prepare and stain a root tip squash to observe the stages of mitosis
 0 purchase

Edexcel A Biology A level core practical Write ups. All include an introduction, hypothesis, variables, method, results, evaluations... CP5 - Prepare and stain a root tip squash to observe the stages of mitosis

Preview 1 out of 4  pages

Document information

  • July 23, 2024
  • 4
  • 2022/2023
  • Exam (elaborations)
  • Questions & answers

Subjects

Written for

All documents for this subject (12)

Seller

avatar-seller
maiaj

Content preview

Core Practical 5 - 18/03/2022


Core practical 5 - prepare and stain a root tip squash to observe the stages of mitosis

Aim : to prepare slides of actively dividing plant tissue, observe the stages of the cell cycle
and determine the duration of the stages of mitosis in relation to the whole cell cycle.

Introduction
Mitosis is the phase of the cell cycle where the nucleus of a cell is divided into 2 nuclei with
an equal amount of genetic material in both the daughter nuclei. It succeeds the G2 phase
and is succeeded by cytoplasmic division after the separation of the nucleus. (Byjus, 2017)

Interphase is known as the preparation for mitosis as it is essential to maintain genomic
integrity. It includes the G1, S and G2 phases during which the cell synthesises new cell
components such as organelles, membranes and new DNA. The formation of new cell
proteins occurs throughout the interphase whereas DNA synthesis occurs only during the S
phase. The individual chromosomes are unravelled to allow access to the genetic material
for new proteins to be synthesised. (University Of York Science Education Group. Nuffield
Curriculum Centre, 2015)

Mitosis is split into 5 stages : prophase, prometaphase, metaphase, anaphase and
telophase. In prophase, the chromosomes condense making them visible under the
microscope and the mitotic spindle begins to form. Then in prometaphase, the nuclear
envelope starts to break down and the microtubules extend from the centromere to attach to
the kinetochores of the chromosomes. (Khan Academy, 2015)

Microtubules that don’t bind to kinetochores can grab on to microtubules from the opposite
pole, stabilising the spindle. More microtubules extend from each centrosome towards the
edge of the cell, forming a structure called the aster. (Khan Academy, 2015)

In metaphase, the chromosomes are lined up along the equator of the cell, then the sister
chromatids are separated from each other and pulled towards the opposite end of the cell in
anaphase. The last stage of mitosis is telophase where two new nuclear membranes form
around each set of chromosomes and the chromosomes begin to decondense. This is
succeeded by cytokinesis, cytoplasmic division, where the cell is pinched by a contractile
ring made of actin filaments. This results in 2 new cells each with a complete set of
chromosomes identical to those of the mother cell. (Khan Academy, 2015)

Staining is a part of histology which allows for the visualisation of tissue structure and
characteristic changes the tissue may have undergone. The histological stains chosen for a
given specimen depends on the investigational question at hand.(Gurina and Simms).
Toluidine blue is an acidophilic metachromatic dye that selectively stains acidic tissue
components and has an affinity for nucleic acids. Therefore it binds to nuclear material of
tissues with high DNA and RNA content.(Sridharan and Shankar)

Hypothesis : We will be able to observe cells and their chromosomes in various stages of
the cell cycle. A lower number of cells will be undergoing mitosis as interphase is the longest
phase of the cell cycle.

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller maiaj. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for £2.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

64257 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy revision notes and other study material for 15 years now

Start selling

Recently viewed by you

Package deal ·

(0)

ALL IN ONE ATI PACKAGE DEAL QUESTIONS AND ANSWERS 2024/2025 GRADED A +BY EXPERTS

Summary ·

(1)

Samenvatting Blok 2.4 Perceptie - Probleem 3

Summary ·

(0)

Volledige samenvatting Inleiding tot de algemene literatuurwetenschap

Exam (elaborations) ·

(0)

BIO 353 Study Guide exam 1 Questions and Answers 100% Pass

Exam (elaborations) ·

(0)

Testbank radiographic pathology for technologists Nina Kowalczyk_ covering all the chapters graded A+

Summary ·

(1)

Samenvatting Wereldoriëntatie 2 AOLB2

Exam (elaborations) ·

(0)

ENGR 201- Final Exam Review short notes from lesson 1- lesson 10 plus final exam questions and answers Sustainable Development and Environmental Stewardship Concordia University

Summary ·

(0)

Detailed Notes of Sustainable Energy - Without the Hot Air

Package deal ·

(0)

Verdiepend arbeidsrecht
£2.99
  • (0)
Add to cart
Added