100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
Summary Passaging Cell Culture Guide £2.99   Add to cart
Quickly navigate to
Preview
  2.  
  3. University Of St Andrews
  4.  
  5. Biology

Summary

Summary Passaging Cell Culture Guide
 12 views  0 purchase

A detailed explanation of how to split cell cultures and why each step is taken. Created by a graduate Biochemistry student who is now studying Biological Sciences at Cambridge University. This is designed to be used for all levels of learning, from GCSE to university. When learning biology at scho...

[Show more]

Preview 1 out of 1  pages

Document information

  • August 6, 2024
  • 1
  • 2024/2025
  • Summary

Subjects

Written for

All documents for this subject (18)

Seller

avatar-seller
susiekleese28

Content preview

Passaging Cells
General Protocol for splitting cell culture


PREPARATION
1 Begin by sterilising the work area and all
equipment in the cell culture hood to maintain a
sterile environment and prevent contamination.

Warm the complete growth medium and trypsin-
EDTA (or trypsin-versene) to 37ºC. This step is
crucial as it prevents temperature shock to the
cells and ensures that trypsin functions effectively


CONFLUENCY
2 Using a microscope, check the confluency of your cell
culture. Cells are typically ready to be passaged when
they reach 70-80% confluency. Overconfluency can
lead to increased cell death due to competition for
nutrients and space.




WASHING
3 Remove the existing culture media from the flask and
wash the cells with a sufficient amount of phosphate-
buffered saline (PBS). Gently rock the flask to ensure
even washing. This step helps to remove debris and dead
cells, which can negatively impact cell health and
experimental results.



DETACH
4 Add the required amount of trypsin-
EDTA/versene to the culture flask. Trypsin is an
enzyme that detaches adherent cells from the
flask surface. Incubate the flask for 5 minutes at
37ºC. After incubation, gently the tap the side of
the flask to dislodge the cells and check under the
microscope to ensure they are in a single-cell
suspension.

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller susiekleese28. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for £2.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

73773 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy revision notes and other study material for 14 years now

Start selling
£2.99
  • (0)
  Add to cart