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MCB 2610 Lab Practical Questions With Answers Graded A+ Assured Success
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  • MCB 2610
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  • MCB 2610

Pertaining to the Gram Stain, cells that retain crystal violet & appear purple are said to be __________ - Gram positive Pertaining to the Gram Stain, cells that do not retain crystal violet & appear pink are said to be Gram ________ - Negative coliform are a subgroup of enteric bacteria classi...

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  • August 23, 2024
  • 5
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • mcb 2610 lab practical

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  • MCB 2610
  • MCB 2610

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MCB 2610 Lab Practical
Pertaining to the Gram Stain, cells that retain crystal violet & appear purple are said to be __________ -
Gram positive



Pertaining to the Gram Stain, cells that do not retain crystal violet & appear pink are said to be Gram
________ - Negative



coliform are a subgroup of enteric bacteria classified by their ability to ferment which sugar? -
lactose



define generation time - the time required for the population to double in number



growth only along the path of the inoculating needle indicates that the bacteria is _______ - non
motile



how is casein hydrolysis detected? - a clear zone is seen around colonies where the casein has
been digested



How to gram stain - label slide

spread sample on slide

allow to air dry

heat fix

flood with crystal violet (1 minute)

wash with Di water

flood with iodine (1 minute)

wash with water

drop Ethanol until no more color comes off

wash with water

flood with safranin (30 sec)

, wash with water

blot dry gently with bibulous paper



in what orientation do you incubate the plates after they have been streaked? - agar half on top



MacConkey agar selects for _______ bacteria - Gram negative

________ will occur around the colonies, indicating amylase production and starch utilization -
clearing zone



motile bacteria use which organelle for propulsion? - flagellum



T/F: a vegetative cell is formed in response to certain environmental conditions such as depletion of
nutrients - False



T/F: brownian motion is the purposeful movement of microorganisms - False



T/F: complex media is made with a precise chemical composition - False



T/F: ethanol is used as a mordant during Gram staining - False



T/F: generation time is calculated in log phase - True



T/F: Gram stains and spore stains are examples of simple stains - False



T/F: heat during staining causes sporulation - False



T/F: heat used during staining does not cause sporulation - True

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