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MediaLab Blood Bank Questions with complete Solutions Graded A+
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MediaLab Blood Bank Questions with complete Solutions Graded A+ - Answers The calculated bleed was 45 mL of fetal whole blood, the dosage calculations would yield 1.5 vials (45 mL fetal whole blood / 30 mL) = 1.5. One vial of Rh immunoglobulin (RhIG) is used for every 30 mL of fetal whole blood (o...

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  • October 9, 2024
  • 29
  • 2024/2025
  • Exam (elaborations)
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  • medialab blood bank

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MediaLab Blood Bank Questions with complete Solutions Graded A+

- Answers The calculated bleed was 45 mL of fetal whole blood, the dosage calculations would yield 1.5
vials (45 mL fetal whole blood / 30 mL) = 1.5. One vial of Rh immunoglobulin (RhIG) is used for every 30
mL of fetal whole blood (or 15mL of fetal packed cells). This number, 1.5, would be rounded to 2 and an
additional vial added. Thus, the final dosage would be 3 vials (900 µg or 4500 IU).

In the interests of safety, if the number to the right of the decimal point is <5, round down and add 1 vial
(e.g., 1.4 = 1 +1 = 2 vials)

If the number to the right of the decimal point is greater than or equal to 5, round up and add 1 vial
(e.g., 1.5 = 2 +1 = 3 vials).

Which of the following steps must be followed to prepare a platelet concentrate?



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Whole blood centrifuged at low speed - plasma separated then centrifuged at low speed

Whole blood centrifuged at high speed - plasma separated then centrifuged at high speed

Whole blood centrifuged at low speed - plasma separated then centrifuged at high speed

Whole blood centrifuged at high speed - plasma separated then centrifuged at low speed - Answers

- Answers In chronically transfused patients, the risk for them developing antibodies against red cell
antigens (RBC alloimmunization) increases by 2% - 8%.

- Answers This field shows an example of true rouleaux. Notice that most of the red cells seen in the
field are stacked together like coins. Four or more cells make up each formation in this slide, leaving
much of the field empty of cells (increased white space). Increased protein in patients with rouleaux
may stain the background blue.



Artificial rouleaux occurs in the thicker part of the slide. When examining a peripheral blood smear, you
should be viewing the red cells in an area where the RBC's are just touching each other in the "feathered
edge" of the slide.



Autoagglutination can occur when a patient develops a cold agglutinin, an autoantibody which reacts
when the red cells are exposed to cold temperatures. The agglutination may be seen on a slide as

,random clumps of red cells. Warming the EDTA blood sample to 37° C will cause the agglutination to
disappear.



Normal RBCs should not be clumped together in the feathered edge of the slide.

Which antibody is associated with Mycoplasma pneumoniae infection and cold hemagglutinin disease?



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Anti-P

Anti-I

Anti-M

Anti-i - Answers M. pneumoniae carries an antigen that resembles I antigen. Thus, when the body
develops an immune response against this antigen, the antibodies may cross react with I antigen on red
blood cells. Anti-I is also associated with cold hemagglutinin disease.



Anti-P may be associated with paroxysmal cold hemoglobinuria.

Anti-M can occur naturally and often reacts at room temperature but is not associated with a specific
disease.

Anti-i is associated with infectious mononucleosis, lymphoproliferative disease, and sometimes with
cold hemagglutinin disease.

A Bombay individual's blood specimen can be differentiated from a blood specimen of a group O person
by which of the following?



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Cells giving a negative reaction with anti-A,B

Testing with anti-H lectin (Ulex europaeus)

Reverse typing with A1 and B cells would be give different reactions

, Testing with Dolichos biflorus - Answers

Which of the following noninfectious complications of blood transfusion is prevented by the irradiation
of blood components?



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Anaphylactic reactions

Febrile non-hemolytic reactions

Transfusion-related acute lung injury (TRALI)

Transfusion-associated graft versus host disease (TA-GVHD) - Answers Irradiation prevents proliferation
of donor T lymphocytes in blood components. T lymphocytes in blood components may cause TA-GVHD
in patients who are immunocompromised, who are receiving components from a blood relative, or who
receive HLA-matched components.

Washed components or components from IgA-deficient donors are indicated for patients at risk for
anaphylactic reactions. The incidence of febrile non-hemolytic reactions has been reduced through the
implementation of universal leukoreduction. One mitigation strategy to reduce the incidence of TRALI is
to collect components from male donors, female donors who have never been pregnant, or female
donors who have been tested since their last pregnancy and are negative for HLA antibodies.

In which of the following cases should Rh Immune Globulin (RhIG) be given?



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Ectopic pregnancy

Rh positive mother carrying an Rh negative fetus

Rh postive mother carrying an Rh positive fetus

Rh negative mother with anti-D formed from previous pregnancy - Answers

Why would a unit of group O blood never be administered to a Bombay patient?



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