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Gel Electrophoresis Questions and Answers Already Passed
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Gel Electrophoresis Questions and Answers Already Passed What type of gel is typically used for separating nucleic acids? Agarose gel is typically used for separating nucleic acids like DNA and RNA. How does the size of DNA fragments influence their migration rate in gel electrophoresis? ...

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  • October 18, 2024
  • 7
  • 2024/2025
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Gel Electrophoresis Questions and
Answers Already Passed

What type of gel is typically used for separating nucleic acids?


✔✔Agarose gel is typically used for separating nucleic acids like DNA and RNA.




How does the size of DNA fragments influence their migration rate in gel electrophoresis?


✔✔Smaller DNA fragments migrate faster through the gel than larger fragments due to less

resistance in the gel matrix.




What is the purpose of applying a dye to the gel before electrophoresis?


✔✔Dyes are used to track the progress of the electrophoresis run and visualize the migration of

the samples.




What kind of current is applied during gel electrophoresis?


✔✔A direct current (DC) is applied during gel electrophoresis to drive the movement of charged

molecules through the gel.




Why is it important to allow the gel to solidify properly before use?


1

, ✔✔Proper solidification ensures that the gel matrix maintains its structure, allowing for accurate

separation of the samples.




What is a potential hazard when using ethidium bromide for staining DNA?


✔✔Ethidium bromide is a mutagen and can intercalate into DNA, posing a potential health

hazard if not handled properly.




How can you determine the success of a restriction enzyme digestion using gel electrophoresis?


✔✔By comparing the band pattern of the digested DNA to a ladder, you can assess whether the

digestion occurred as expected.




What effect does voltage have on the resolution of DNA fragments during electrophoresis?


✔✔Higher voltage can speed up the run but may reduce resolution, while lower voltage can

improve resolution but take longer to separate fragments.




Why is it necessary to include a control sample when running gel electrophoresis?


✔✔Including a control sample helps validate the results by providing a standard for comparison

and confirming that the gel worked properly.




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