Gel Electrophoresis Lab Questions and Answers Rated A+
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Module
Gel Electrophoresis Lab
Institution
Gel Electrophoresis Lab
Gel Electrophoresis Lab Questions and
Answers Rated A+
What happens when DNA enters the gel—does it have a “map” to follow?
DNA doesn’t need a map; it travels through the gel based on its size, zipping toward the
positive end as it follows the electric current.
How does the gel f...
Gel Electrophoresis Lab Questions and
Answers Rated A+
What happens when DNA enters the gel—does it have a “map” to follow?
✔✔DNA doesn’t need a map; it travels through the gel based on its size, zipping toward the
positive end as it follows the electric current.
How does the gel feel like a “party” for DNA fragments?
✔✔The gel is like a crowded party where smaller DNA fragments can weave through the crowd
easily, while larger ones struggle to get through the door.
What’s the secret ingredient in the buffer that keeps DNA comfortable during its journey?
✔✔The buffer solution contains salts and a stable pH that creates a friendly environment,
ensuring the DNA moves smoothly without getting “stressed out.”
Why might the DNA fragments act like children at a race?
✔✔In gel electrophoresis, smaller fragments are like sprinters who dash ahead, while larger ones
might take their time, trying to squeeze through a crowded course!
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, What role does the loading dye play in making DNA feel “fashionable”?
✔✔The loading dye adds color to the DNA samples, making them stylish as they enter the gel,
and helps them stand out on their journey.
What could it mean if your gel looks like a messy paint splash instead of neat bands?
✔✔A chaotic, smeared gel could mean there was too much DNA, or the run was too fast—like
everyone got too excited at the party!
What’s the magical trick that lets you see your DNA after electrophoresis?
✔✔The magic comes from dyes like ethidium bromide that bind to DNA and glow under UV
light, transforming invisible fragments into shining stars!
How does the DNA ladder act like a coach at a race?
✔✔The DNA ladder gives your samples something to compare against, helping you gauge how
well your “runners” performed on the gel track.
What kind of “fitness test” does gel electrophoresis perform on DNA?
✔✔Gel electrophoresis tests how well DNA fragments separate based on size, revealing which
“athletes” are strong and speedy and which are lagging behind.
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