Gel Electrophoresis Diagram Latest Update 100% Pass
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Gel Electrophoresis Diagram
Institution
Gel Electrophoresis Diagram
Gel Electrophoresis Diagram Latest
Update 100% Pass
What is the purpose of the wells at the top of the gel?
The wells are used to load DNA samples into the gel before the electrophoresis process
begins.
Why are the DNA samples loaded near the negative end of the gel?
DNA is negatively c...
Gel Electrophoresis Diagram Latest
Update 100% Pass
What is the purpose of the wells at the top of the gel?
✔✔The wells are used to load DNA samples into the gel before the electrophoresis process
begins.
Why are the DNA samples loaded near the negative end of the gel?
✔✔DNA is negatively charged, so it is loaded near the negative end so it can migrate toward the
positive end.
What do the bands on the gel represent?
✔✔The bands represent DNA fragments that have been separated based on their size.
Why do smaller DNA fragments appear farther down the gel?
✔✔Smaller DNA fragments travel faster through the gel and, therefore, move farther from the
wells than larger fragments.
What is the function of the power source in gel electrophoresis?
1
, ✔✔The power source generates an electric current that moves the DNA through the gel.
Why does the DNA move toward the positive end of the gel?
✔✔DNA is negatively charged, so it is attracted to the positively charged electrode at the far end
of the gel.
What is the purpose of the buffer solution in the electrophoresis chamber?
✔✔The buffer conducts electricity and helps maintain a stable pH, ensuring smooth movement
of DNA through the gel.
Why are there multiple bands visible for a single DNA sample?
✔✔Each band represents a fragment of DNA of a specific size, separated from the others during
electrophoresis.
What does the distance between bands indicate about DNA fragment size?
✔✔The farther apart the bands, the greater the difference in size between the DNA fragments.
How can you tell if the DNA has successfully migrated across the gel?
2
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