The complete summary notes of the different microscopes and their uses. The summary includes advantages and disadvantages of all types which is commonly asked in past exam questions. Microscopes including SEM, TEM ,light and laser confocal.
Microscopy
* resolution the
ability to individual
objects separate entities
-
see as
size
image
I
magnification calculation : -Image
size
actualsin ATM
a
magnifica
melve : Im
im : Mo Electron Microsy
um : 1x106 i
nm (x109
:
* in
light microscopy magnification ,
can be increased easily however resolution is
a
limiting factor
teelection microscopy <Inm is used to illuminate the species detail of the cell
- beam
of electing / a -
ultrastructure/beceen as the h elections : a tran
light
Disadvantages :
↳ expensive
↳ must be trained to use it
Le can
only be used in a specic environment
↳ specimen could be damaged electron beam
by
↳ specimen cannot be alive - must be placed in a vacuum before. : must
already be dead
↑ ransmission electron microcaps (TEM) :
↳ beam
of elections are transmitted tre a
specimen + focussed to produce an
image ->
best resolution
↳ electrons pass turn contrast
dense parts deasily creating a
↳ nat image = 20
↳ possible magnification up to X2 million
↳ resolving power O Sum
.
&
Scanning Electron Micnecope (SEM)
minWe e
↳ elections are collected it
beam
of elections are sent across the surface
of a specimen
and reflected
↳ resolving power 3-10 mm so resolution not TEM
as good as the
↳ elems bounced
if sample
↳ final image 3D
↳ possible magnification is x200 , 00
Laser Scanning Confocal microscopy :
An light intensity is used to illuminate a specimen which has been treated w/ florescent de
↳ final
image is coloured
* can use tuck samples/while organisms
↳ the Laser is reflected by the dyes
* ↑ resolchen
used
*
by medics as it is a non-invasive method
↓ than election
* expensive
* cmage 3D
final
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