Other
Unit 2 Learning Aim C
- Institution
- PEARSON (PEARSON)
I achieved a Merit in this assignment.
[Show more]
Content preview
Mariya HussainGroup 3
10632164
Unit 2
7/03/2023
Unit 2C: Using Chromatographic techniques to identify components in
mixtures.
What is Chromatography?
Chromatography involves a solution being passed through a media
where the constituent elements of the mixture dissolves at different
speeds to allow the dissolved compounds to be separated from a
mixture.
Practical report for paper chromatography and TLC
Paper chromatography – allows low-molecular-mass compounds to be
separated based on their arrangement of the stationary and mobile
phase. I drew 4 points at the bottom to mark each solution for Histidine,
Glycine, tyrosine and the unknown sample. I added each of the samples
to the correct point and added solution 3 and air dried it. I then placed it
onto a uv light where I marked any spots that had appeared and circled
it. I then used a ruler to measure how far the solvent had travelled to the
solvent line and added all results on a graph. I interpreted these results
to find out what the Rf values were for each of the 4 samples. Large
amounts of samples would ruin the results.
TLC- separates non-volatile mixtures. I drew 4 points at the bottom to
mark each solution for Histidine, Glycine, tyrosine and the unknown
sample. I added each of the samples to the correct point and added
solution 3 and air dried it. I then placed it onto a uv light where I marked
any spots that had appeared and circled it. I then used a ruler to
measure how far the solvent had travelled to the solvent line and added
all results on a graph. I interpreted these results to find out what the Rf
values were for each of the 4 samples. Results that are found is difficult
to reproduce.
, Stationary phase of Paper chromatography: water trapped between the fibres in the paper.
Stationary phase of TLC: thin coating of absorbent solid on a sheet of plastic or glass. Silica,
alumina or cellulose.
Mobile phase of TLC: ethanol and NH3
Mobile phase of paper chromatography: ethanol and NH3
Chromatography solvent: butan-1-ol: ethanoic acid: water
Paper chromatography and TLC are both examples of qualitative analysis. Paper
chromatography can be revealed using UV light or by developing the chromatogram by
revealing it to a gas or vapour which gives the compound colour when it is reacted with paper.
TLC determines in a mixture the number of components which allows 2 substances to be
identified or it monitors the reaction’s progress.
In both TLC and Paper chromatography, the substance that is present in the chromatography is
shown by how intense the coloured spot is.
Thin Layer Chromatography of amino acids
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller mariyahussain2020. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £4.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
53068 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy revision notes and other study material for 14 years now