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Summary Types of Microscopes

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Summary of types of microscopes

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  • January 1, 2025
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  • 2015/2016
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Types of microscopes
First review @September 12, 2023

Practise Q's Done


Transmission Electron Microscope (TEM)
a beam of electrons is transmitted through a specimen

the beams are focused to produce an image of the specimen

TEM’s have a magnification of x5,000,000 and a resolution of 0.1nm

microscope has very good quality

TEM’s can only view dead organisms

this is because electron microscopes rely on staining to create a contrast so that the
specimen can be viewed in more detail and so that organelles and cells can be
distinguished and identified

Scanning Electron Microscope (SEM)
a beam of electrons are sent across the surface of a specimen, rather than being
transmitted through them

this is called topography

only an image of the outside of the cell is produced

SEM’s have a magnification of x5000 and a resolution of 10nm

SEM’s can also only view dead organisms because they are a type of electron microscope

Laser Scanning Confocal Microscope
a single spot of light is moved across a specimen

the parts of the specimen that are stained with a dye will emit fluorescent light which will
be detected

this reflected light from the microscope is focused onto the detector by a confocal
pinhole

only the light radiated from the distance that gives the sharpest focus is detected




Types of microscopes 1

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