BIOC1302 Experiments Biochemistry (BIOC1302)

Worksheets + Answers of Experiments performed during Year 1 Biochemistry. To separate molecules on the basis of size difference. Your learning objectives · To understand the practical and theoretical basis of molecular exclusion chromatography. · To understand how columns are set up and run. · To understand the terms 'bed volume' and 'void volume' and how they are measured. · To be able to apply simple tests to identify the eluted proteins. · To appreciate some applicatio...

To determine the activation energy for an enzyme catalysed reaction • To determine whether the thermal denaturation of trypsin is entropy or enthalpy driven

To purify α-lactalbumin from milk whey, using affinity chromatography. To identify the proteins in cow’s milk whey To determine the purity of α-lactalbumin separated from milk whey

Purifying proteins by ion exchange chromatography Aims of the experiment • To separate two proteins using difference in net charge. Your learning objectives • To understand the practical and theoretical basis of ion exchange chromatography through its application to protein separation. • To understand how columns are set up and run. • To be able to use the knowledge to devise separation procedures using alternative ion exchange resins. • To appr...

MEASUREMENT OF SERUM CHOLESTEROL USING A FLUORESCENCE ASSAY

Understanding the use of buffers in Biological sciences, answered worksheet (pH...etc)

How to make the titration curve of glycine and process data regarding this. Also showing principal concepts of: pH, PKa