2d chromatography - Study guides, Revision notes & Summaries

ACS Biochemistry First Semester Exam Questions with All Correct Answers Buffer Order and Henderson Hasselbach - ANSWER Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - ANSWER Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - ANSWER a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - ANSWER 3.6 Supe...

ACS Biochemistry Comps EXAM QUESTIONS WITH COMPLETE SOLUTIONS Ion-Exchange Chromatography - ANSWERS Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used to remove stuck proteins. Hydrophobic/Reverse Phase Chromatography - ANSWERS Beads are coated with a carbon chain. Hydrophobic proteins stick better. Elute with non-H-bonding solvent (acetonitrile). Affinity Chromatography - ANSWERS A...

Footwear Evidence - answers-Different shoes will leave behind prints of different sizes and patterns Visible 2-D footprint - answers--Dirt of material picked up by shoe or tire and deposited onto flat, hard surfaces. -Variety of surfaces, materials, and details -Collection: examination quality photo Visible 3-D footprint - answers--Shoeprint made in soft material (dirt, snow) -Composition of dirt or snow will determine the detail of the impression. -Collection: make a cast (ex. Plaster...

Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - ANSWER Separates sample based on size with smaller molecules el...

ACS BIOCHEMISTRY EXAM 2023 Q&A With complete solution Updated Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - ...

ACS BIOCHEMISTRY EXAM 2023 Q&A With complete solution Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - ANSWER Se...

ACS BIOCHEMISTRY EXAM 2023 Q&A With complete solution Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - ANSWER Se...

ACS BIOCHEMISTRY EXAM Q&A With complete solution Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - ANSWER Separates sa...

ACS BIOCHEMISTRY EXAM Terms in this set (252) Henderson-Hasselbach Equation pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Separates sample based on size with ...

1. Henderson-Hasselbach Equation: pH = pKa + log ([A-] / [HA]) 2. FMOC Chemical Synthesis: Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. 3. Salting Out (Purification): Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. 4. Size-Exclusion Chromatography: Separates sample based on size with small- er molecules eluting later. 5. Ion-Exc...