Bradford assay - Study guides, Revision notes & Summaries

UF BACE Practice missed Qs Questions and Answers Fully Solved Which of the following should be used to calculate the amount of compound required to make a solution? Molecular weight Carbohydrates Example: cellulose. Functions: energy storage, receptors, structure of plant cell wall. Lipids Example: cholesterol. Functions: membrane structure, energy storage. The enzyme maltase catalyzes the break down of maltose into glucose. A technician added maltase to a solution of ma...

Hemocytometer-How to use Correct Answer 9 boxes loaded with 10 ul 1 box = 1 ul Multiply by 1000/1000 to obtain the value of cells/ml Multiply that value by the total volume of the cell suspension to obtain an accurate number of cells in suspension Hemocytometer- What is it? Correct Answer A hemocytometer is a simple technique for not only determining the cell density of a solution but for experimental applications such as tracking cell growth in a solution over time and medical applic...

BACE Exam Questions and Answers Latest Updated The enzyme maltase catalyzes the break down of maltose into glucose. A technician added maltase to a solution of maltose to be incubated at 37°C for 20 minutes. Samples were taken from the reaction tube every five minutes, and the glucose and maltose concentrations were measured. Which of the following is an appropriate negative control for this experiment? Maltose incubated with water What is the best definition of the term "hypothesi...

The Bradford assay only works at low protein concentrations (from 0.05 to 0.5 mg/mL protein) How can we reduce the concentration of protein in our sample? - Answer dilute the sample what is the volume range of the P1000 - Answer 200 uL - 1000 uL how would you set the volume to pipette 20 uL with the P20? - Answer "200" how much protein do you need to add to the 800 uL buffer in the first microcentrifuge tube to make a 1:5 dilution? - Answer 200 uL how much fluid from th...

Accuracy a measure of how close a result matches a correct or standard value. For example, how close a dart is to the bullseye in a dartboard is an indicator of accuracy. Precision a measure of variability. For example, three darts placed close together on a dartboard indicate high precision, but if this cluster of darts is far from the bullseye it would also indicate low accuracy. Brainpower Read More Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute ...

Safety in the lab 1. Wear gloves 2. Wear goggles 3. Wear close toed shoes 4. Tie up long hair 5. No water or food types of micropipettes P20, P200, P1000 Micropipette an instrument used to measure/deliver amounts of liquid with high accuracy and precision. to find dilution (sample + diluent)^2 To calculate how much solvent and solute to mix together to create a new solution at lower concentration C1V1=C2V2 C1V1 C1= stock solution (mg/mL) V1= volume of the st...

BIO 111 TAMU lab exam 1 latest 2023 with certified solutions Accuracy a measure of how close a result matches a correct or standard value. For example, how close a dart is to the bullseye in a dartboard is an indicator of accuracy. Precision a measure of variability. For example, three darts placed close together on a dartboard indicate high precision, but if this cluster of darts is far from the bullseye it would also indicate low accuracy. an ideal experiment yields results with both high ac...

Electromagnetic (EM) spectrum visible light electromagnetic energy is classified based on the wavelength highest energy= gamma radiation the spectrophotometer is used to measure the specific wavelengths and the quantity of light absorbed by a substance the extent to which a solution absorbs light is called the absorbance higher absorbance value equals darker solution the absorbance of a solution is found by using Beer's Law, A=Ebc a measure of how well the substance absorbs light at th...

What is the macromolecule concentration that is being measured in the Bradford Assay lab (lab 1)? - ANSWER-Protein concentration is measure in the lab. The name of the protein is BSA or Bovine Serum Albumin. diluent - ANSWER-solution - usually buffer or water - with which the sample is diluted. aliquot - ANSWER-A portion of a well-mixed sample removed for testing (a sub amount of the total. dilution factor - ANSWER-how diluted a solution is compared with the starting solution of same co...

Absolute configuratoin the nomenclature system used for the three dimensional arrangement of atoms in isomers; the most common systems are D/L and R/S R= clockwise S= counter clockwise D= right clockwise L= counter clockwise acetal Brainpower Read More 0:10 / 0:15 acetly CoA an important metabolic intermediate that links glycolysis and beta oxidation to the citric acid cycle; can also be converted into ketone bodies activation the conversion of a biomolecule to i...