Dna extraction - Study guides, Revision notes & Summaries

SUCCESS! in Clinical Laboratory Science - Molecular Diagnostics (26-50) Exam Graded A+ For the purpose of diagnosing genetic diseases, what component of whole blood is used for the extraction of DNA? A. Leukocytes B. Plasma C. Platelets D. Red blood cells - answerA. Leukocytes A. Leukocytes are routinely used for extraction of DNA from human blood. Mature red blood cells and platelets have no nuclei. Plasma or serum can be used for detection of viremia, but it is not used for analysis...

Molecular diagnostics: fundamentals methods and clinical applications: ch 4 Molecules that make up DNA backbone - ANS-Ribose Sugar and phosphate nitrogen base bound to an unphosphorylated sugar - ANS-nucleoside starting source of organic and inorganic DNA isolation - ANS-cells in suspension Four general steps of organic DNA isolation - ANS-lysis, acidification, phenol chloroform extraction, DNA precipitation Three general steps for inorganic DNA isolation - ANS...

A micoarray is testing for: - Answer a lot of genetic characteristics from one sample Southern Hybridization - Answer extract DNA probe w/ DNA Northern Hybridization - Answer extract RNA probe w/ DNA Western Hybridization - Answer extract proteins probe with antibodies What is the first method that became standard in DNA sequencing? - Answer Sanger method What type of PCR does Sanger Method use? - Answer cycle sequencing PCR Cycle Sequencing PCR requirements -...

Molecular Diagnostics Chapter 3 Exam 100% Solved Which sample types must be amplified prior to analysis? - answerbuccal cells, saliva, bone, teeth, hair, dried blood, fixed tissue What are two ways to break down cell walls for DNA extraction? - answer1. homogenization through physical grinding 2. treatment of detergent (SDS) with strong base (NaOH) in ethylene- diaminetetraacetic acid (EDTA) Ficoll - answerHighly branched sugar Solution w/ specific density that allows only RBCs and neut...

MB (ASCP) 100% VERIFIED SOLUTIONS LATEST UPDATES what does topoisomerase do? - ANSWERbreaks phosphate backbone to "un" supercoil DNA Another name for Topoisomerase II - ANSWERGyrase Helicase - ANSWERUn"zips" DNA at replication fork What makes up the primosome? - ANSWER1. primase 2. helicase 3. DNA C NASBA stands for... - ANSWERnucleic acid sequence based amplification NASBA - ANSWERRNA is target RNA-->cDNA Reverse transcriptase (RNA-->cDNA) followed by DNA dependent ...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - answerminiprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - answerCesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - answerMiniprep. Which of the following wavelengths is incorrectly matched? - answer240 nm - RNA concentration. The ...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - answerminiprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - answerCesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - answerMiniprep. Which of the following wavelengths is incorrectly matched? - answer240 nm - RNA concentration. The ...

MB ASCP Full Questions and Answers 100% correct What are the two categories of manual nucleic acid extraction liquid phase extractions Solid-support extractions What chemicals are included in liquid phase manual nucleic acid extraction phenol/chloroform What are solid-support manual nucleic acid extractions based on reversible associations between nucleic acids and silica matrix column packing, or magnetic beads functionalized with surface chemistries specific for the nucleic ...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 In a phenol-chloroform extraction, DNA will be in the aqueous phase - answerWhich is true regarding DNA purification? (a) In a phenol-chloroform extraction, DNA will be in the aqueous phase (b) the most common way to purify plasmid DNA is a cesium chloride gradient (c) the best way to purify DNA is via anion exchange chromatography (d) DNA w/ an A260:A280 of 1.25 is pure (e) a miniprep will produce -1 mg of DNA PolyTs - answe...

Molecular Diagnostics Chapter 3 Exam 100% Solved Which sample types must be amplified prior to analysis? - answerbuccal cells, saliva, bone, teeth, hair, dried blood, fixed tissue What are two ways to break down cell walls for DNA extraction? - answer1. homogenization through physical grinding 2. treatment of detergent (SDS) with strong base (NaOH) in ethylene- diaminetetraacetic acid (EDTA) Ficoll - answerHighly branched sugar Solution w/ specific density that allows only RBCs and neut...