2d chromatography Study guides, Revision notes & Summaries

Exemplar assignment for the final assignment of Unit 2 in BTEC Applied Science, Unit 2D, which is about the skills that you've developed over the course of Unit 2 from all of the experiments. This assignment was awarded a DISTINCTION. Please don't hesitate to send me a message if you have any questions about this assignment!

- Refresh - Introduction to proteomics - Sample prep - Amino-acid analysis - 2D-page - High pressure liquid chromatography - Mass spectrometry: instruments - Tandem mass spectrometry -Mass spectrometry: applications - Methods for quantification

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - answerSeparates sample b...

ACS Biochemistry First Semester Exam With Complete Solutions 2024 Buffer Order and Henderson Hasselbach - answerWater, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - answerΦ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - answera: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - answer3.6 Super secondary structure - answerhelix turn helix...

Biochemistry Exam #2 (Module 3) questions and answers 100% verified. (Exam question 21) Dialysis - correct ates small molecules from large molecules (Exam question 30) When a protein is 'salted-in', it precipitates and is removed form a solution. - correct answers.False When we purify protein, we are going against the natural tendency toward disorder (we are fighting against entropy). - correct answers.True When centrifuging sub-cellular structures, what organ...

ACS Biochemistry First Semester Exam With Complete Solutions 2024 Buffer Order and Henderson Hasselbach - answerWater, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - answerΦ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - answera: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - answer3.6 Super secondary structure - answerhelix turn helix...

ACS Biochemistry First Semester Exam With Complete Solutions 2024 Buffer Order and Henderson Hasselbach - answerWater, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - answerΦ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - answera: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - answer3.6 Super secondary structure - answerhelix turn helix...

ACS Biochemistry First Semester Exam Questions with All Correct Answers Buffer Order and Henderson Hasselbach - ANSWER Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - ANSWER Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - ANSWER a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - ANSWER 3.6 Supe...

Buffer Order and Henderson Hasselbach - Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - 3.6 Super secondary structure - helix turn helix parallel vs. antiparallel - parallel wedges face same way antiparallel face opposit...

Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - ANSWER Separates sample based on size with smaller molecules el...