Fluorescence assay - Study guides, Revision notes & Summaries

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

CAPS421 Midterm Test Questions and Answers Autophagy - Answer-Process of degrading cytosolic content in double membrane vesicles Defective autophagy - Answer-Impaired autophagy leading to protein accumulation and reduced myelin degradation Autophagy activators - Answer-Strategies to enhance autophagy in defective cases Proficient autophagy - Answer-Functional autophagy that preserves metabolic fitness and resistance to hypoxia and cancer treatment Autophagy inhibitors - Answer-Stra...

MIP 300 Final exam questions with 100% correct answers who provides the lipids in a viral envelope - ANSWERS the cell all viruses have a protein coat - ANSWERS true a virulent phage can inadvertently package host cell chromosome into phage particles - ANSWERS true you have a bacterium that picks up a new gene by Hfr conjugation. what is an explanation of why progeny of this bacterium express this trait? - ANSWERS the gene is integrated into the genome after successful transformation of ...

QBM Final Exam Questions With 100% Correct Answers What is the definition of Molecular biology - answerStructure and function of macromolecules (proteins and nucleic acids) What is the central dogma of DNA? - answerDNA ⟶ RNA ⟶ Proteins What BSL corresponds to minimal hazards? - answerBSL-1 What does BSL-1 correspond to? - answerminimal hazards What BSL corresponds to organisms with potential hazards? - answerBSL-2 What BSL(s) require proper protective equipment? - answerBSL2-4 Under...

Molecular Diagnostics Exam Which of the following determines the sensitivity of an immunoassay? a. avidity of the antibody b. specificity of the antibody c. affinity of the antibody e. none of the above - answerc. affinity of the antibody Monoclonal antibodies provide immunoassays: a. low initial costs b. broad reactivity c. shorter lead times d. lot to lot consistency - answerd. lot to lot consistency Which of the following characterizes a capture enzyme assay? a. best for high-mole...

QBM Final Exam Questions With 100% Correct Answers What is the definition of Molecular biology - answerStructure and function of macromolecules (proteins and nucleic acids) What is the central dogma of DNA? - answerDNA ⟶ RNA ⟶ Proteins What BSL corresponds to minimal hazards? - answerBSL-1 What does BSL-1 correspond to? - answerminimal hazards What BSL corresponds to organisms with potential hazards? - answerBSL-2 What BSL(s) require proper protective equipment? - answerBSL2-4 Under...

ASCP MLS Module Test With Complete Solutions What condition has the highest stringency for DNA probe hybridization? High temperature, low salt concentration What additive can interfere with the efficiency of polymerase chain reaction (PCR)? Sodium heparin What molecule marker is diagnostic for a coagulopathy? Factor V R506Q Panel A represents a typical native result for a real-time PCR test to detect cytomegalovirus CMV in plasma. The test includes primers and fluorescein amid...

QBM Final Exam Questions With 100% Correct Answers What is the definition of Molecular biology - answerStructure and function of macromolecules (proteins and nucleic acids) What is the central dogma of DNA? - answerDNA ⟶ RNA ⟶ Proteins What BSL corresponds to minimal hazards? - answerBSL-1 What does BSL-1 correspond to? - answerminimal hazards What BSL corresponds to organisms with potential hazards? - answerBSL-2 What BSL(s) require proper protective equipment? - answerBSL2-4 Under...

MIP 300 Final examwho provides the lipids in a viral envelope - correct answer the cell all viruses have a protein coat - correct answer true a virulent phage can inadvertently package host cell chromosome into phage particles - correct answer true you have a bacterium that picks up a new gene by Hfr conjugation. what is an explanation of why progeny of this bacterium express this trait? - correct answer the gene is integrated into the genome after successful transformation of a frag...

FRNSC 421W - EXAM 1 PRACTICE QUESTIONS AND ANSWERS (100% PASS) RFLP - Answer️️ -restriction fragment length polymorphism - DNA digested by restriction enzymes at repeat sequences, yields different fragment lengths for different people - Gel electrophoresis to separate fragments by size, MW ladder for reference - Southern blotting to physically transfer DNA to membrane - Radioactively labeled probe hybridizes with immobilized DNA on membrane - Exposure to X-ray film yields carbon copy...