Plasmid - Study guides, Class notes & Summaries

Core Promoter Elements ~~> TATA box = repetitive sequence of thymine and adenine Initiator (INR) = sequence of pyramidines like thymine and cytosine DPE = downstream promoter elements found along the DNA Common to have >1 promoter but not all of them CpG islands ~~> Sequences high in CG frequency. They are able to escape methylation which would lead to gene silencing. Demethylation would lead to over expression of genes. Regulatory Promoter Elements ~~> UAS and URS f...

Biology 1402- Test 1 Questions and Answers Already Passed A community plus its physical environment is a/an ________. A) thallus B) ecosystem C) population D) plasmid ecosystem The ultimate source of energy for nearly all life on Earth is the sun; plants and certain other organisms convert solar energy into chemical energy by the process of _________. A) the citric acid cycle B) cellular respiration C) photosynthesis D) glycolysis photosynthesis ___________ includes chan...

Microbiology Lab Final Exam Questions and Answers Already Passed What is the primary function of the bacterial cell wall? A) Energy production B) Protein synthesis C) Protection and shape maintenance D) DNA replication C) Protection and shape maintenance What is the purpose of the streak plate method in microbiology? A) To test antibiotic susceptibility B) To isolate individual bacterial colonies C) To identify bacterial species D) To perform a Gram stain B) To isolate indiv...

Vertical gene transfer is the process of genes being passed down from parent to offspring Correct Answer-True When bacteria take up naked genetic material from the surrounding environment, this is called Correct Answer-transformation When one bacterium transfers a copy of its plasmid to another bacterium, this is called Correct Answer-conjugation Transformation. transduction. and conjugation are all examples of Correct Answer-horizontal gene transfer What is the name of the process o...

Micr 290 Final Exam and Predictor (WEST COAST UNIVERSITY) Exam Newest 2025 Actual Exam Complete Verified 250 Questions and Detailed Answers(VERIFIED ANSWERS) GRADED A+ What is cloning? – Correct Answer-Cloning refers to the process of inserting a sequence of DNA (also called an insert) into a plasmid. The resulting plasmid is a form of recombinant DNA, meaning that it is an artificial (i.e. laboratory-produced) construct combining DNA sequences from two or more different sources....

CMMB 343 Exam Study Guide with 100% Correct Answers | Verified | Latest 2024 Version How does phi6 penetrate the host cell? - because it is an enveloped phage, it uses the entry and uncoating model that relies upon spike-protein mediated endocytosis - once in the vesicles, it releases the viral particles and proteases digest the capsid coat, lysing the vesicle, and releasing the phage genome into the cytoplasm Which protein is the plug at the end of the tail tube in T4, preventing DNA from...

transduction Correct Answer The mechanism of DNA transfer in bacteria where DNA is transferred by phages (bacterial virus) nicks at specific origin, outer strand peels off and goes through pillis to F- cell while being resynthesized in F+ Correct Answer What is the process of conjugation? F- cells (replicate the F plasmid so the F- cell has one too) Correct Answer For conjugation, what do F+ cells look for? 0.13 to 14 million bp, e coli has 4.6 million bp Correct Answer What is the ...

BiOL 3301 - Exam 2 Questions And Answers A+ 2025 Bacteria do possess allelic differences that affect their cellular traits - ANS-However, these allelic differences (such as different sensitivity to antibiotics) are between different strains of bacteria because: - Bacteria are usually haploid - Bacteria reproduce asexually Bacteria are usually haploid - ANS-This fact makes it easier to identify loss of function mutations in bacteria than eukaryotes Bacteria reproduce asexually - ANS...

Bacteria Structure Capsule, outer membrane (only in gram-negative bacteria), cell wall murein, cytoplasmic membrane, nucleus equivalent, plasmid, 70S ribosomes, depot substances (metaphosphates, glycogen), flagella, attachment pili Gram-Positive Bacteria Thick peptidoglycan membrane (cross-links between NAM + peptide and NAG)

All of the following are characteristics that make plasmids good cloning vectors, except A. they are small B. they are well characterized C. they can be transferred into appropriate host cells D. they can carry genetic markers, such as antibiotic resistance genes E. they can accept a relatively large amount of foreign DNA Ans: E. they can accept a relatively large amount of foreign DNA Types of Plasmids: -Fertility Factors -Resistance Factors -Bacteriocin Factors -Virulence Plasmids ...