Plasmid - Study guides, Class notes & Summaries

You want to clone a DNA fragment that has been digested with BamHI into a plasmid vector. What should you do to prevent religation of the vector? If you don't do this what other problems will occur? Correct Answer-To prevent religation you should treat it with alkaline phosphatase which will knock off the 5' phosphate. As a result you will have 5' and 3' OH which means ligase can't act on it. If you don't, you could get a concatemer or the insert could go in upside down or backwards. ...

ASCP Molecular Biology Certification Detailed Exam With Questions and Answers Well Answered A+ Graded 100% Excellence. Do you have to know the gene sequence in order to do DNA sequencing? - CORRECT ANSWER-Yes, in order to design primers You do NOT need to know the mutation Pyrimidine - CORRECT ANSWER-One carbon ring Cytosine, Thymine, Uracil Purine - CORRECT ANSWER-Two carbon rings Adenine, Guanine How are nucleotides joined together? - CORRECT ANSWER-Condensation to form phosphodie...

BIOE 206 Exam 1; Questions and Answers 100% Correct Plasmid DNA purification steps Correct answer-1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and genomic DNA 7. put the suspension on a spin column 8. plasmid DNA will adhere to spin column and everything else will be washed away with high salt buffers 9. add low salt buffer to remo...

BIOE 206 Exam 1 UPDATED ACTUAL Questions and CORRECT Answers Plasmid DNA purification steps - CORRECT ANSWER- 1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and genomic DNA 7. put the suspension on a spin column 8. plasmid DNA will adhere to spin column and everything else will be washed away with high salt buffers 9. add low salt ...

A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features except: A) a selectable antibiotic marker such as kanamycin resistance. B) a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice. C) a Ti plasmid lacking its T-DNA segment. D) active vir gene products from the altered Ti plasmid. E) an ability to induce crown gall formation in infected leaves. - Answer-B) a shuttle vector with 25 bp TDNA repeats ...

BMD 330 Exam 2 Questions & Answers 2025/2026 rifamycins, actinomycin D - ANSWERSdrugs that inhibit protein synthesis erythromycin, spectinomycin, chloramphenicol, aminoglycosides - ANSWERSdrugs that interfere with the ribosome conjugation - ANSWERSdonor cell with pilus, fertility plasmid in donor, both donor and recipient alive, bridge forms between cells to transfer DNA Direct conjugation - ANSWERScan transfer drug resistance, resistance to metals, toxin production, enzymes, adhe...

WGU C652 Exam Review (Latest 2024/ 2025 Update) Heredity and Genetics | Questions and Verified Answers| 100% Correct| Grade A Q: chemical modifications of nucleotides Answer: Chemical mutagens can modify bases in DNA and alter their base-pairing properties Q: tetraploidy Answer: containing four homologous sets of chromosomes Q: trisomy Answer: a condition in which an extra copy of a chromosome is present in the cell nuclei, causing developmental abnormalities...

UMD General Biology 2 Exam 3 UPDATED ACTUAL Exam Questions and CORRECT Answers Are archaea and bacteria prokaryotic or eukaryotic? - CORRECT ANSWER - prokaryotic Are eukarya prokaryotic or eukaryotic? - CORRECT ANSWER - Eukaryotic important features of bacteria - CORRECT ANSWER - no membrane bound organelles, circular chromosomes, plasmid DNA, cell wall, plasma membrane, cytoplasm, ribosom

MBIO 404 Question and answers rated A+ DNA can be sheared by sonication, the use of sound energy. Answer choices True or False - correct answer True When using an E. coli. expression vector, it is not possible to predict whether the recombinant protein product will fold properly. - correct answer True The Ti plasmid of Agrobacterium tumefaciens? allows colonization of plant cells and use of plant resources. is introduced to plants via a protozoan vector. allows this ba...

loop of Henle correct answers where Na+ is reabsorbed from the filtrate moving through the nephron This is ultimately the mechanism whereby the kidneys concentrate urine - section of the nephron tubule that conserves water and minimizes the volume of urine action potentials result in correct answers an increased permeability of the plasma membrane to sodium - Na+ in causes depolarization hyperglycemia promotes cellular dehydration because correct answers glucose molecules raise the osmo...