CHE 4220 Exam 2 || Already Graded A+.

immunological assay method correct answers What method do we use when we want to find a protein we are interested in but we don't know the exact genetic sequence? antibody binding correct answers What mechanism does the immunological assay method use to highlight the desired protein? primary antibody correct answers In the immunological assay method, the ____ binds to the target protein. secondary antibody correct answers In the immunological assay method, the ____ reacts with the protein of interest and causes a visible change. functional complementation correct answers Screening DNA for ____ helps identify its physiological role. cap, untranslated regions, codons, poly A tail correct answers Mature eukaryote mRNA has four main regions, what are they? cap correct answers The ___ helps a eukaryotic mRNA strand attach to the ribosome and protects from exonuclease activity. poly A tail correct answers The ___ helps a eukaryotic mRNA strand export from the nucleus and protects the mRNA. oligo dT beads attach to poly A tail correct answers While cloning eukaryotic sequences, how is the mRNA isolated from the total RNA? reverse transcripticase converts mRNA to single strand cDNA correct answers While cloning eukaryotic sequences, after the mRNA is isolated, what happens next? RNaseH degrades mRNA correct answers While cloning eukaryotic sequences, after the cDNA is formed, what happens next? DNA polymerase forms second strand of cDNA correct answers While cloning eukaryotic sequences, after RNaseH degrades mRNA, what happens next? 10 kb correct answers Plasmids can handle inserted DNA as large as...? bacteriophage, cosmid correct answers What are some examples of high capacity vectors? virus that destroys bacteria correct answers What is a bacteriophage? 10-20 kb correct answers How many base pairs can bacteriophage lambda carry?