Dna probes - Study guides, Class notes & Summaries

Microbiology Question and answers correctly solved Fungi Cells Eukaryotic internal organelles Cell walls = complex carbs / Chitin + Glucan + Mannose protein - Glucan is a Pharm target for Fungi - Also contain Ergosterol Yeast Single-celled fungi Replicate by budding Buds = Blastoconidia Tested for using: Morphology and Biochem - API 20C AUX / DNA Techniques Monomorphic Filamentous Fungi (Mold) Fungi with Hyphae (filamentous cells) - Cluster of Hyphae = Mycelium - Septate H...

HUMAN GENETICS (BMD 350) EXAM I TEST BANK 2024/ACTUAL 700 EXAM QUESTIONS WITH 100% CORRECT AND VERIFIED ANSWERS WITH EXPLANATIONS/A+ GRADE ASSURED To visualize chromosomes, chromosomes must be: - Answer-extracted, stained with dyes and DNA probes Genetics - Answer-The study of inherited traits and their variation What notable event occurred in 1859? - Answer-Charles Darwin hypothesized pangenesis, and termed gemmules

FISH - Cells fixed in place Fluorescently labeled probes consisting of 165 rRNA gene sequence sent to attach to RNA View in Epifluorescence microscope Cultivation independent methods - DNA from unculturable bacteria can be amplified and sequenced by PCR Sequences can be used to produce fluorescent probes that will bind to complementary DNA (Fluorescent In Situ Hybridization FISH) Microbial consortia - Microbes that can't grow on their own Metagenomics - DNA is isolated from an envir...

Virology and Mycology Viruses - correct answer Obligate intracellular parasites Require living mammalion cells to grow and multiply They use the cells replication structures to reproduce They are released by buddig from, leaking out of, or rupturig the host cell Virology Labs - correct answer Keep several different types of cell cultures to grow viruses for identification Require a significant amount of lab space Most labs do not provide full service Usually provide rapid virology te...

EXAM 2: MCB 2610 Exam 2 with Complete Solutions Cultivation independent methods CORRECT ANS-DNA from unculturable bacteria can be amplified and sequenced by PCR Sequences can be used to produce fluorescent probes that will bind to complementary DNA (Fluorescent In Situ Hybridization FISH) FISH CORRECT ANS-Cells fixed in place Fluorescently labeled probes consisting of 165 rRNA gene sequence sent to attach to RNA View in Epifluorescence microscope

ASCP MB: Extra-Practice Questions & Answers 2024 The Klenow fragment of E. coli DNA polymerase I is used: - ANSWER-to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - ANSWER-gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucle...

The pathophysiology of Glazmann thrombasthenia is: A. Deficiency of dense granules B. Deficiency of glycoprotein Ib/IX receptor C. Deficiency of glycoprotein IIb/IIIa receptor D. deficiency of microtubules - C. Deficiency of glycoprotein IIb/IIIa receptor Chagas disease is caused by: A. Trypanosoma cruzi B. Trypanosoma palladium C. Trypanosoma brucei D. None of the above - A. Trypanosoma cruzi What would a patient with a microcyctic/hypochromic anemia most likely have noted on their CB...

Cultivation independent methods - DNA from unculturable bacteria can be amplified and sequenced by PCR Sequences can be used to produce fluorescent probes that will bind to complementary DNA (Fluorescent In Situ Hybridization FISH) FISH - Cells fixed in place Fluorescently labeled probes consisting of 165 rRNA gene sequence sent to attach to RNA View in Epifluorescence microscope Metagenomics - DNA is isolated from an environmental sample and sequenced Metagenomic information must stil...

SAEM M4 Exam GRADED A {LATEST} PID cause - CORRECT ANSWER originates as a cervical infection with Neisseria gonorrheaand/or Chlamydia trachomatis, and becomes polymicrobial as it ascends into the uterus, fallopian tubes and ovaries. 3 sx PID - CORRECT ANSWER -lower abd pain -purulent vag d/c -vag bleed when getPID sx - CORRECT ANSWER Symptoms begin shortly after the start of the menstrual cycle, when there are fewer defenses by the cervical mucosal barrier to ascending infections. ...

What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction enzymes 2. Fragments are separated by gel electrophoresis 3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), complementary to region of interest What is RFLP? - ️️- Restriction Fragment Length Polymorphism - The number and location of restriction sites for a given restriction enzymes are not the same in all individuals What is the mapping difference between linear DNA f...