Cmb2001 Study guides, Class notes & Summaries

CMB2001 Exam Questions With Verified Answers What is a promoter? - answerDNA sequence which RNA polymerase can bind to and transcribe the protein Prokaryotic promoters - answerTATAAT -(10 region) 'Pribnow box' TTGACA - (-35 region) - two consensus sequences Prokaryotic RNA polymerase - answer1 RNA Polymerase makes all 3 kinds of RNA Composed of 5 subunits, four comprise the core enzyme What is the core enzyme? - answerHelps to initiate and synthesize RNA. 4 main polypeptides (2 alpha...

©THEBRIGHTSTARS 2024 CMB2001 Exam Questions With Verified Answers. Describe how H3Lys9me heterochromatin is formed - answerH3Lys9me heterochromatin formation: Lys9 deacetylated by HDAC. Suvar39 then methylates lys9. Methylated lys9 is recognised by HP1 and then HP1 binds. HP1 has chromodomain - chromodomains often recognise and bind to methylated lys residues. Chromodomain on HP1 is specific for H3Lys9me (with di or tri methylated lys 9). Binding of HP1 is thought to compact nucleosome a...

CMB2001 Exam Questions With Verified Answers. What is gene expression? - answerdsDNA is decoded to ssRNA/proteins by an mRNA intermediate using A/U/C/GTPs and RNA polymerase Where is the consensus sequence in prokaryotic promoters? - answerat -10 and -35 upstream of the the transcription start site What recognises the consensus sequences in prokaryotes? - answerthe holoenzyme What is a holoenzyme? - answera closed complex with a core enzyme and sigma factor 70 What does the holoenzyme do...

CMB2001 Exam Questions With Verified Answers. Core Promoter Elements - answerTATA box = repetitive sequence of thymine and adenine Initiator (INR) = sequence of pyramidines like thymine and cytosine DPE = downstream promoter elements found along the DNA Common to have >1 promoter but not all of them CpG islands - answerSequences high in CG frequency. They are able to escape methylation which would lead to gene silencing. Demethylation would lead to over expression of genes. Regulatory...

CMB2001 Exam Questions With Verified Answers In prokaryotes RNA polymerase requires what co-factor? - answer70 sigma factor Forms the holoenzyme The promoter is formed of two hexameric sequences at what positions? - answer-35 and -10 The amount of product transcribed is dependant on how close the promoter sequences are to what? - answerConsensus sequence Eukaryotic promoters have a basal region which can contain a TATA box, initiator (Lnr), BRE (TFIIV recognition element) and what other ...

CMB2001 – 9 Exam Questions With Verified Answers. 21 - answerhow many proteins are in small subunit of the 70S ribosome 33 - answerhow many proteins are in small subunit of the 80S ribosome 31 - answerhow many proteins are in large subunit of the 70S ribosome 50 - answerhow many proteins are in large subunit of the 80S ribosome prokaryotes - answer23S and 5S rRNA make up the large subunit of the ribosome in ...... eukaryotes - answer28S, 5.8S and 5S rRNA make up the large subunit of the...

CMB2001 L01 Exam Questions With Verified Answers. What is the span of the core/basal region of the eukaryotic promoter? - answer-30 to +30nt What elements are in the core region of the eukaryotic promoter? - answerTATA box Inr (initiator) DPE (downstream promoter element) BRE (TFIIB recognition element) Describe the TATA box consensus sequence - answer-31 to -26nt, TATAWAWR Describe the Inr sequence - answer-2 to +4nt, YYANWYY with an A at +1 What is observed in core promoter CpG island...