Rflp - Study guides, Class notes & Summaries

ASCP MB 2023/2024 E. coli RNA Polymerase II - Answer DNA template, product: mRNA RNA Polymerase I - Answer DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - Answer DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - Answer DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - Answer DNA template, product: mRNA Mammalian DNA polymerase α - Answer DNA template, product: primers ...

Which of the following polymorphisms are tandem repeats of 1-8 bp? a. Single nucleotide polymorphisms b. Variable number tandem repeats c. Short tandem repeats d. Restriction fragment length polymorphisms → Short tandem repeats Which of the following is used as a marker of gender? a. D8S1179 b. Amelogenin c. TPOX d. Penta D → Amelogenin What is an expression for how many times more likely the child's allele is inherited from the alleged father than by random occurren...

ASCP MB Final Exam Practice Questions with Correct Answers 2023 (Already Graded A+) Lining up 2+ sequences to search for maximal regions of identity in order to assess extent of relatedness/homology Bioinfo: Local alignment - Answer Alignment of some portion of 2 sequences Bioinfo: Multiple sequence alignment - Answer Alignment of 3+ sequences arranged with gaps so that common residues are aligned together Bioinfo: Optimal alignment - Answer Alignment of 2 sequences with best degree of ide...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Chapter 13 - DNA Purification, Agarose Gel Electrophoresis, and DNA Visualization - answer The genetic information of all living organisms is contained within the sequence of ____ ____ = Examples include: purines and pyrimidines = For most organisms, the genetic information is contained in nucleic acids of the deoxyribonucleic acid (DNA), or ribonucleic acid (RNA) in certain viruses - answernucleic acids ______ is a complete s...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Chapter 13 - DNA Purification, Agarose Gel Electrophoresis, and DNA Visualization - answer The genetic information of all living organisms is contained within the sequence of ____ ____ = Examples include: purines and pyrimidines = For most organisms, the genetic information is contained in nucleic acids of the deoxyribonucleic acid (DNA), or ribonucleic acid (RNA) in certain viruses - answernucleic acids ______ is a complete s...

What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction enzymes 2. Fragments are separated by gel electrophoresis 3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), complementary to region of interest What is RFLP? - ️️- Restriction Fragment Length Polymorphism - The number and location of restriction sites for a given restriction enzymes are not the same in all individuals What is the mapping difference between linear DNA f...

(MB) ASCP TEST PREP COAGULOPATHIES - -Disorder of blood coagulates caused by inherited or acquired defects in coagulation proteins, platelets, or vasculature -Von Wildebrand's Disease, hemophilia, Factor V Leiden DECREASE EXPRESSION - -Methylation of cytosine bases 5' to the gene -siRNAs complementary to the gene transcript INCREASE EXPRESSION - -Histone acetylation close to the gene PRADER-WILLI - -Type of mutation: deletion on chromosome 15 -Gene location: 15q11.2 -Sequence: trans...

FRNSC 421W - EXAM 1 PRACTICE QUESTIONS AND ANSWERS (100% PASS) RFLP - Answer️️ -restriction fragment length polymorphism - DNA digested by restriction enzymes at repeat sequences, yields different fragment lengths for different people - Gel electrophoresis to separate fragments by size, MW ladder for reference - Southern blotting to physically transfer DNA to membrane - Radioactively labeled probe hybridizes with immobilized DNA on membrane - Exposure to X-ray film yields carbon copy...

What is metagenome? - correct answer Sampling the genome sequence of a community of organisms. Polymorphism involved for Irinotecan? - correct answer UGT1A1 NGS related to deletions and insertions, coverage, depth? - correct answer -Paired-end reads for detection of indels -Coverage is how much of your target has been read -Depth is how many times a...

Purpose of this lab - correct answer Analyze our own DNA to see what form of TAS2R38 (taster gene): heterozygous, homozygous (dominant or recessive) What did we use to extract DNA from us? - correct answer Saline solution Importance of saline solution - correct answer Salt neutralizes the negative charge of the sugar phosphate backbone of DNA --> caus...