Mip 302 - Study guides, Class notes & Summaries

MIP 302 Exam 1 Questions with All Correct Answers What is viral cultivation? - Answer-The replication of a virus in living cells What is the main necessary component of viral cultivation? - Answer-Living cells Describe the setup of the plaque assay. - Answer-E. coli is mixed with a bacteriophage in a TSA then poured into a plate; the E. coli form a bacterial lawn then the phages infect the E. coli cells and lyse them, forming the plaques Why did you dilute the phage stock in the pla...

MIP 302 Exam 1 Questions with Revised Answers What are some main mechanisms of chemotherapeutic agents? - Answer-1. inhibition of cell wall synthesis 2. inhibition of protein synthesis 3. inhibition of nucleic acid synthesis 4. cell membrane disruption 5. metabolic antagonism In general, how do penicillin and other β-lactam antibiotics work? What cell types are the most effective against? Why are they not harmful to humans? - Answer-they target cell wall synthesis and we do not hav...

MIP 302 Final Test Questions with All Correct Answers What can the API test strip identify? - Answer-Gram negative rods Why is water placed in the bottom of the API test strip? - Answer-Because the media is small it will dry up making tests hard to decipher What does an underlined well mean on an API strip? - Answer-Fill tube and add oil What does a boxed well mean on an API strip? - Answer-Fill Tube and cupule with bacteria What does a well that is not underlined or boxed mean? ...

MIP 302 Exam 2 Questions with All Correct Answers Why are capture ELISAs also called sandwich ELISAs - Answer-they are called sandwich ELISAs because the target antigen is "sandwiched" between the 2 antibodies What two components are common to all ELISAs? - Answer-Every ELISA will have either a target antigen or a capture antibody bound to a solid matrix. It will also have a reporter system. How does blocking help to inhibit false positive results? - Answer-blocking inhibits false po...

MIP 302 - ExamHow do you calculate total magnification? - correct answer Objective Magnification x Ocular Magnification = Total Magnification How do you calculate resolving power? - correct answer RP = (0.5 x Wavelength)/Numerical Aperture What is resolving power? - correct answer A microscope's ability to distinguish between two objects that are very close together What is the best resolving power for viewing microbes? - correct answer 0.2 um What is numerical aperture? - correct a...

MIP 302 FINAL EXAM Lab VII: Define Eubacteria: - correct answer True bacteria Lab VII: Archaebacteria - correct answer ancient bacteria (can survive in extreme environments) Lab VII: Thermophiles - correct answer live in env. greater than 55 C or 131 F Lab VII: Hyper(super) thermophiles - correct answer live in high temp. env > 150 C or 300 F Lab VII: Psychrophiles - correct answer live in cold temp. 15 C or 59 F Lab VII: Acidophiles - correct answer live in...

MIP 302 - Exam 2 Where is the ocular lens located? - correct answer Where is the arm of the microscope located? - correct answer Where is the objective lens located? - correct answer Where is the diaphragm of the microscope located? - correct answer Where is the fine adjustment knob located? - correct answer Where is the coarse adjustment knob located? - correct answer Where is the light source of the microscope? - correct answer Where is the stage of the microscope?...

MIP 302 - Exam 1 2024 with Answers to Questions

What bacteria inhabit extreme environments? correct answers often archaebacteria, or "ancient" bacteria How does archaebacteria differ from eubacteria or "true" bacteria? correct answers they differ in cell wall and cell membrane composition, ribosome composition and shape, tRNA structure and RNA polymerase sequences What are thermophiles? correct answers bacteria that inhabit environments with elevated temperatures What are hyper or super thermophiles? correct answers bacteria that...

MIP 302 Exam Prep Questions with Verified Answers