Plasmid Study guides, Class notes & Summaries

Test Bank for Genetics A Conceptual Approach, 7th Edition Benjamin Pierce Test Bank for Chapter 2: Chromosomes and Cellular Reproduction Multiple-Choice Questions 1. Which of the following statements is false? a. Errors in chromosome separation are rarely a problem for an organism. b. Errors in chromosome separation can result in a miscarriage. c. Errors in chromosome separation can result in cancer. d. Errors in chromosome separation can result in a child with severe handicaps. e. Erro...

Exam 3: BIOS 242 / BIOS242 Review (Latest 2024/ 2025 Update) Fundamentals of Microbiology with Lab| Questions and Verified Answers| 100% Correct| Grade A- Chamberlain Q: what is an antigen? foreign molecule that causes a specific immune response Q: what is another name for antigens? Answer: immunogens Q: where do antigens come from? Answer: proteins and polysaccharides from cells and viruses Q: what are the functions of plasmid cells? Answer: release antibodies...

Bacterial Transformation Lab Report Title: Bacterial transformation lab Objective(s): The length of the time for which the heat shock is given greatly affects the process of transformation or the uptake of the plasmid by the bacterial cell. Hypothesis: As the length of the time of the heat shock increases there are greater chances of the process of transformation or the uptake of the plasmid by the bacterial cell. As the time period of heat shock increases, the bacterial cell membrane deve...

MICROBIOLOGY EXAM 4 (PRACTICE QUESTIONS) WITH COMPLETE SOLUTIONS 100% All of the following are generally used in vaccines EXCEPT... a. toxoids b. parts of bacterial cells c. live, attenuated viruses d. inactivated viruses e. antibodies - correct answer e. antibodies A reaction between an antibody and soluble antigen-forming lattices is called a(n)... a. agglutination reaction b. complement fixation c. immunofluorescence d. neutralization reaction e. precipitation reaction. -...

BIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% Correct Plasmid DNA purification steps - ANSWER-1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and ...

A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features except: A) a selectable antibiotic marker such as kanamycin resistance. B) a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice. C) a Ti plasmid lacking its T-DNA segment. D) active vir gene products from the altered Ti plasmid. E) an ability to induce crown gall formation in infected leaves. - Answer-B) a shuttle vector with 25 bp TDNA repeats ...

You want to clone a DNA fragment that has been digested with BamHI into a plasmid vector. What should you do to prevent religation of the vector? If you don't do this what other problems will occur? Correct Answer-To prevent religation you should treat it with alkaline phosphatase which will knock off the 5' phosphate. As a result you will have 5' and 3' OH which means ligase can't act on it. If you don't, you could get a concatemer or the insert could go in upside down or backwards. ...

BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED Methylation: a primitive immune system for bacteria - hds-R - Answer-- plasmid transforms into cells are protected (restriction enzyme not expressed) Methylation: a primitive immune system for bacteria - mcrA/mcrB/mrr - Answer-degrades foreign DNA that is not properly methylated - can occur to DNA obtained from mouse/human cells containing CpG methylated DNA (can interfere with cloning of mammalian genomic DNA) pCR 2.1 - TOP...

What is antibiotic selection? Correct Answer-Survival of a population of antibiotic resistant cells within a larger population of antibiotic sensitive cells. Where can antibiotic selection occur? Correct Answer-Anywhere where there are antibiotics and bacteria present. The best-surviving bacteria will be selected for. Name a common use for antibiotic selection in microbiological research. Correct Answer-To identify and isolate cells that have piece of DNA of interest such as a particular p...

BIOE 206 Exam 1; Questions and Answers 100% Correct Plasmid DNA purification steps Correct answer-1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and genomic DNA 7. put the suspension on a spin column 8. plasmid DNA will adhere to spin column and everything else will be washed away with high salt buffers 9. add low salt buffer to remo...

WGU C652 Exam Review (Latest 2024/ 2025 Update) Heredity and Genetics | Questions and Verified Answers| 100% Correct| Grade A Q: chemical modifications of nucleotides Answer: Chemical mutagens can modify bases in DNA and alter their base-pairing properties Q: tetraploidy Answer: containing four homologous sets of chromosomes Q: trisomy Answer: a condition in which an extra copy of a chromosome is present in the cell nuclei, causing developmental abnormalities...