Plasmid Study guides, Class notes & Summaries

BIO 205 Exam Questions With 100% Correct Answers Nucleoid is a region/structure found only in ____ cells. a) prokaryotic b) eukaryotic c) bacterial d) Both A and C Prokaryotic/bacterial A plasmid is double-stranded DNA. True or false? True A plasmid-gene or a chromosomal-gene (piece of DNA with a specific product) will always produce a________. a) carbohydrate b) ATP c) Protein d) Lipid Protein DNA polymerase is a(n) a) lipid b) polysaccharide c) enzyme d) nucelic ...

transduction Correct Answer The mechanism of DNA transfer in bacteria where DNA is transferred by phages (bacterial virus) nicks at specific origin, outer strand peels off and goes through pillis to F- cell while being resynthesized in F+ Correct Answer What is the process of conjugation? F- cells (replicate the F plasmid so the F- cell has one too) Correct Answer For conjugation, what do F+ cells look for? 0.13 to 14 million bp, e coli has 4.6 million bp Correct Answer What is the ...

IB Biology semester 1 Latest Version Graded A+ Cells Smallest functional unit of an organism What are the principles of cell theory? 1. All living things are made of cells 2. Cells are smallest unit of life 3. Cells come from pre-existing cells What are the properties of life? Metabolism Reproduction Growth Response Excretion Homeostasis Nutrition Prokaryotic cells Single cell which has neither a distinct nucleus or organelles can be an organism (e.g. bacteria) Flage...

WSU Bio 102 Final Exam Questions and Answers Already Passed How does BPA cause cancer? it disrupts meiosis and mitosis causing breast and prostate cancer. 3 and 7 plastics may contain BPA What is a genetic fingerprint? how do you make one? -Genetic Fingerprint: DNA uses the differences in base sequences to identify individuals. -DNA fingerprinting involves 3 tools: 1. PCR to get large amounts of DNA 2. Restriction digest with blunt ends to fragment the DNA with precision 3. Gel...

what is antibiotic selection? Correct Answer-the most resistant cells surviving when antibiotic resistance is around where can abx selection occur? Correct Answer-anywhere that bacteria and abx is present name a common use for abx selection in microbiological research Correct Answer-identifying particular cells that contain a DNA of interest -ex: plasma, transform cells w a plasma and plate them on an agar Why does virtually every plasmid used for scientific research contain an antibio...

Name the main organelles in an animal cell: - ANSWER > nucleus > cytoplasm > cell membrane > ribosomes > mitochondria What organelles does a plant cell have but animal cell doesn't? - ANSWER > chloroplast > cell wall > vacuole What organelles does a Bacteria cell have? - ANSWER > no nucleus > circular strand of DNA > rings of plasmid > cell wall > cell membrane

Core Promoter Elements ~~> TATA box = repetitive sequence of thymine and adenine Initiator (INR) = sequence of pyramidines like thymine and cytosine DPE = downstream promoter elements found along the DNA Common to have >1 promoter but not all of them CpG islands ~~> Sequences high in CG frequency. They are able to escape methylation which would lead to gene silencing. Demethylation would lead to over expression of genes. Regulatory Promoter Elements ~~> UAS and URS f...

Nucleoid is a region/structure found only in ____ cells. a) prokaryotic b) eukaryotic c) bacterial d) Both A and C - Answer-Prokaryotic/bacterial A plasmid is double-stranded DNA. True or false? - Answer-True A plasmid-gene or a chromosomal-gene (piece of DNA with a specific product) will always produce a________. a) carbohydrate b) ATP c) Protein d) Lipid - Answer-Protein DNA polymerase is a(n) a) lipid b) polysaccharide c) enzyme d) nucelic acid - Answer-c- enzyme DNA transcri...

Bacteria Structure Capsule, outer membrane (only in gram-negative bacteria), cell wall murein, cytoplasmic membrane, nucleus equivalent, plasmid, 70S ribosomes, depot substances (metaphosphates, glycogen), flagella, attachment pili Gram-Positive Bacteria Thick peptidoglycan membrane (cross-links between NAM + peptide and NAG)

Marasmus vs. Kwashiorkor MEALS protein? correct answers Marasmus-low in both calories and protein (no specific vitamins or mineral deficiency), NO edema Muscle wasting (wrinkly skin) Kwashiorkor-severe protein deficiency --> skin lesions and edema (small child big abdomen) Low serum albumin --> Low serum oncotic pressure Liver fatty change due to decrease apolipoprotein synthesis and eposition Malnutrition Edema Anemia Liver (fatty) Skin lesions (hyperkeratosi...