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Summary AQA GCSE Biology (Part of) Topics 1 to 5 £3.39   Add to cart
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Summary AQA GCSE Biology (Part of) Topics 1 to 5
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contains notes on: - culturing microorganisms - effect of antibodies on bacterial growth - monoclonal antibodies - uses of monoclonal antibodies - the brain - investigating the brain - the eye - how the eye focuses on near and distant objects - long and short-sightedness - plant diseases ...

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  • Topics 1 to 5
  • April 29, 2021
  • 12
  • 2020/2021
  • Summary

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Culturing Microorganisms Agar Gel Plate

Need

Petri dish
contains nutrient broth
Ager gel

On ager gel plate bacteria grow divisible colonies




Preparing Culture MUST prevent contamination

1 sterilise all petri dishes bacterial nutrient broth agar
kills
any unwanted microorganisms
2 Sterilise inoculating loop by passing it through Bunsenflame
then transfer bacteria into culture


3 attach lid of Petri dish with adhesive tape
stops lid from falling unwanted microorganisms entering

4 place agar plate UPSIDE DOWN into incubator

stops moisture dripping down onto bacteria disrupting
colonies




Temperature

C
in school labs normally incubate bacteria at 25

reduces chances of harmful bacteria growing

, Effect of antibiotics on Bacterial Growth

1 clean bench with disinfectant solution

kills microorganisms that can contaminate culture


2 sterilise inoculating loop by passing it through Bunsen
flame

Bunsen flame
3 open a sterile agar gel plate near


kills bacteria in air


4 spread chosen bacteria over plate use loop
evenly
5 place sterile paper discs containing antibiotic onto plate
incubate plate at 250C



AFTER A FEW DAYS

bacteria shouldie formed layer over
agar gel
around antibiotic discs region where bacteria have not
grown
called zone of inhibition


Measure effect of antibiotic

of inhibition
by calculating area of Zone

use tr

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