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APPLIED SCIENCE – BTECHCHROMATOGRAPHY
HOW TO START?
EXPLAIN WHAT CHROMATOGRAPHY IS
AND HOW IT WORKS? WHAT IS USED
FOR? WHAT IS USED TO SEPARATE?
EXPLAIN THE PHASES
ALSO EXPLAIN DIFFERENT TYPES OF
CHROMATOGRAPHY USED FOR
SEPERATION
FOR MORE DEATIL ON NEXT PAGE
(PURCHASE)
GO ON EXPLAINING IN DEATIL YOUR
EXPEIREMENT – ON HOW TO AIM THIS
PURCHASE
USING THE EQUATION MEASURE THE R/F
VALUE FROM THE CHROMATOGRAM FOR
MORE – PURCHASE
, APPLIED SCIENCE – BTECH
WHAT IS CHROMATOGRAPHY?
Chromatography can be used to
separate components in a mixture. Both
quantitative (measureable) and
qualitative (non-numerical) data can be
obtained.
HOW IT WORKS?
• Chromatography is used to separate
mixtures of substances into their
components. All forms of
chromatography work on the same
principle.
• They all have a stationary phase (a
solid, or a liquid supported on a solid)
and a mobile phase (a liquid or a gas).
The mobile phase flows through the
stationary phase and carries the
components of the mixture with it.
Different components travel at different
rates.
TYPES
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