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Samenvatting Verbetering van alle oefeningen van centrifugatie van het vak scheiding en zuivering van biomoleculen (BMW: behaald resultaat 18/20) £5.56   Add to cart

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Samenvatting Verbetering van alle oefeningen van centrifugatie van het vak scheiding en zuivering van biomoleculen (BMW: behaald resultaat 18/20)

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Verbetering van alle oefeningen van centrifugatie van het vak scheiding en zuivering van biomoleculen (BMW: behaald resultaat 18/20): Dit is een samenvatting van het vak Scheiding en Zuivering van Biomoleculen gegeven door Xaveer Van Ostade. Het bevat een volledige verbetering van alle oefeningen v...

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  • November 3, 2021
  • 3
  • 2021/2022
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OEFENINGEN CENTRIFUGATIE:

Oefening 1

Drie groepen:

Bereken de RCF bij een centrifuge met een maximale snelheid van 5233 rps op een partikel
dat zich op 47 mm van het rotatiecentrum bevindt.




RCF = 2.r/g = 1.12 . 10-5 . r . (y rpm)2 s2.cm-1

RCF = 1.12 . 10-5 . 4,7 . (50.000)2 rpm2 s2 . cm . cm-1

RCF = 131.600 rpm2 s2

Dus RCF = 131.600 g

Zelfde berekeningen voor 77 (= 215.176 g) en 107 mm (= 299.010 g).

Vergelijk met figuur III.1.2.


De berekening kan ook rechtstreeks vanuit rps gemaakt worden. In dat geval is de
omrekeningsformule niet nodig en kan onmiddellijk uit de formule RCF = 2.r/g berekend
worden:

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