This is my Unit 2C assignment. I spent lots of time and effort on this and have achieved a DISTNICTION from this. If you have any questions, enquires or need some assistance please feel free to send me a message. Good luck with your assignment!
Chromatography
(See https://www.britannica.com/science/chromatography for where I got my
information.)
The term chromatography can be described as a technique used to separate the
compounds within a mixture (or solutes), determined by the relative proportions of
each solute distributed between a moving fluid stream, which is called the mobile
phase, with it also containing a mobile phase that tends to be quite contagious. The
, stationary phase might be in the form of a solid or a liquid whereas during the mobile
phase that can be represented in the states of either liquid or gas.
How does the process of paper chromatography work?
The method of chromatography happens when either a fluid or gas is passed over a
filter material. While the atoms are being transported through the filter, they are
inclined to scatter. The instrument of division is dependent upon the method utilized to
filter the molecules, this in any case is decided by the assortments of particles. Once
the particles partition over the filtering material; they travel at dissimilar speeds, which
comes about with the atoms unwinding and more often than not, appearing to contain
an assorted coloured line across the filtering material.
One of the key things to keep in mind is that bigger particles tend to travel more
gradually while smaller atoms travel much quicker. Meaning that the particles drop out
of cadence as they travel at distinctive speeds.
During the course of this assignment, I will be conferring the bunch of methods of
chromatography; paper chromatography of extracted of plant pigments and thin layer
chromatography (TLC) of extracted plant pigments as well as amino acids.
Paper Chromatography of plants
There are two stages when carrying out paper chromatography: the mobile phase and
the contiguous stationary phase. With the mobile phase is a solvent while the
stationary phase is the paper. The separation occurs when the compound splits, reliant
on the various characteristics of each individual compound. (see
https://chemdictionary.org/paper-chromatography/ )
The stationary phase:
Paper chromatography uses paper, which is made up of cellulose, with this being a
polymer of simple sugar (glucose). Cellulose contains a -OH resulting in the paper
being very polar. In this manner permitting the compounds to make Hydrogen bonds
and interact utilizing Van Der Waals and Dipole-Dipole forces.
Factors affecting paper chromatography
Quality of paper utilized during the practical: There are a variety of papers which
are used when doing paper chromatography, due to this some classes of paper are
better suited to either absorbing or retaining the results of the spot moving upwards,
however some paper makes it more challenging for the solvent dot to progress
upwards.
Type of solvent used: Due to each compound having a different solubility this results
in them all having different effects of separation on the paper chromatography paper.
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