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Lecture notes

BS5110 Research and Career Development

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The lectures are given here. The first is on research literature, then data analysis, statistical analysis, PCR, HPLC, brief other protein biochemistry techniques, CV, proposals, and ethics.

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  • March 30, 2023
  • 26
  • 2020/2021
  • Lecture notes
  • Dr. semerdjieva
  • All classes
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Introduction 3/2/2021
Timetable: all sessions online.
Assessments: Project proposal 1800 words. News article on research at UEL is based
on solid ethical principles 400 words (one page A4; explained to Newham borough). CV,
group interview, and poster presentation is the other component. You are assigned jobs
for the group interview. Employability session is CV and linkedin profile, and interview
talk. Then you submit the CV. 28 April is your interview, then they discuss what should
be improved on CVs and how the interview went. Poster presentation-when you go into
interviews sometimes you need some sort of presentation (like lecturer at UEL you pass
a test and have PhD, then prepare a session where you teach, then interview where
they ask you to prepare a presentation).
Vitro-in cell lines. Vivo-in living animals. Knockout, knockin, knockdown. Trp53 tumor
suppression.
Research Literature Group Poster Presentation Assessment
Types of Research Literature: Primary articles - original research articles, case
reports/case series, and technical notes. Secondary articles - narrative review articles
and systematic reviews. Special articles - letters to the editor, correspondences, short
communications, editorials, commentaries, and pictorial essays. Tertiary – aimed at lay
audience or researchers from a different area of knowledge; found in science
magazines, newsletters. Articles in newspapers, encyclopedias, online. Gray literature.
Scientific Literature: PubMed – often free full-text archive of biomedical and life
sciences journal literature at the U.S. National Institutes of Health’s National Library of
Medicine (NIH/NLM). Google Scholar - search across many disciplines and sources:
articles, theses, books, abstracts and court opinions, from academic publishers,
professional societies, online repositories, universities and other web sites. arXiv - is an
open-access repository (maintained at Cornell) where you can freely download and
read pre-print research papers from many quantitative science areas. Quantitative
Biology: Biomolecules; Cell Behaviour; Genomics; Molecular Networks; Neurons and
Cognition; Other Quantitative Biology; Populations and Evolution; Quantitative Methods;
Subcellular Processes; Tissues and Organs. UEL library -
https://uelac.sharepoint.com/sites/libraryandlearningservices. Social media. Friends and
colleagues.
Peer review process: acts as a filter to ensure that only high-quality research is
published by determining the validity, significance and originality of the study; improves
the quality of manuscripts because peer reviewers provide suggestions to authors on
how to improve the quality of their manuscripts and identify any errors that need
correcting before publication.
Impact Factor: The impact factor (IF) measures the frequency with which the average
article in a journal has been cited in a particular year. It is a measure of the importance
or rank of a journal. Calculated by citations in a year divided by the number of

,publications for the past two years. The higher the number, the higher the impact factor
so higher ranking the journal is.
Poster Content: Title. Introduction – introduce the subject with the significance of the
topic; background information; the aim/ objectives of the poster: what the poster is going
to address; best done with bullet points and a diagram. Methods – brief description of
experimental design – flow chart, timeline. Results – present data acquired in a logical
manner - telling a story. Discussion – main points, challenges or gaps of information.
References – bottom right; small font. Citations throughout the text – small font.
Dos and Donts: Need to have a graphic hierarchy related to importance. Simple graphs
and tables-they tell more than paragraphs. Figure and tables need legends (above
tables, below figures) and should be numbered. Small font for the source of
figure/tables. NO pictures just to impress (this is a scientific poster-small fly compare
size to other organism, do not show a picture! There should be a purpose. Figures and
tables should be discussed or referred to in the poster (in the text AND presentation-as
you can see in figure, it shows _). No video needed; look how you want. Use
reasonable amount of colour/ background. Avoid heavy colours (if poster is to be
printed) - be friendly to the environment. So, a good poster is easy to read; delivers a
clear message; and has an organised structure. 40 pts bold is scientific standard, visual
hierarchy. Figures take most of the poster.
Papers allocated in week 3 for the poster.
Group poster presentation task: Groups of 3 students organised within the module
groups as discussed in the Introduction to BS5110. Papers allocated in Week 3 during
the Discussion sessions on original research papers. You have to imagine that the data
in the paper is your own and that you are going to a meeting to present some of that
data. Group members organise themselves to work as a team and produce a poster
and deliver successfully the presentation. Details in following slides.
Poster Preparation: We are providing you with a Power Point template (A3 size) which
you can download from Moodle – Assessment and Feedback section. The template is
only an aid, you can modify as you please but you should maintain the main items: Title,
Background, Methodology, Results, Discussion/Conclusion, Citations in text and
References at the end (use smaller font to save space). Keep the text to a minimum.
The writing must be your own and not copied from the paper.
Title (on template): You can use the original title of the paper you are using In this box
also enter the name of the original authors and their institutions and the journal in which
it was published.
Introduction: To keep text to a minimum use a self-made diagram to summarise the
content of the Introduction. On the template: Provide a brief description of general
background of the project and an explanation the rationale of the study. Include the
Aims of the project. Preferably use bullet points, keep text short. Use a diagram that
you have drawn or produced using PowerPoint or any other suitable software.

, Materials and Methods: Produce a flow chart summarising the experimental design
rather than describe the methods in paragraphs. Template says: Brief description of the
experimental plan (not the detailed methods). Use a flow diagram.
Results: Produce one figure using the data in the paper; other figures may be used as
they are in the paper. Template: Presentation of the results in a visual form (rather than
text). Figures and graphs illustrating the results say much more than words! Can be
organized in different panels. Present them in a logical way. You should: 1) Produce
one figure based on the data in the paper; 2) Select any other figures from the paper
that are relevant to your presentation. Figure: Figure legend should be self-explanatory
and help reader understand what is presented in the figure. Smaller font, but readable.
Discussion/Conclusion: If it is more suitable to express the conclusions with a
summary figure, then one should also be produced. Template: Conclusion drawn from
analysis of the results. It is the “TAKE HOME” message of the poster. Highlight it above
the rest. If a diagram will illustrate the take home message better, make and present
one.
References (on template) If you have cited other pieces of work in the poster (i.e. in the
introduction), the full citation should be included in the poster. This can be in a small
font.
Poster Delivery: One student from a group will submit the pdf file containing the group
poster to a TurnItIn link. The PDF will allow you to pan over the sections which you are
describing during the presentation. The lecturers marking the presentations will share
the screen showing your submitted poster. Each student in the presentation group
should present for ca. 3 minutes. You will have a maximum of 8 min to present the
whole poster. Each student will be asked at least one question on content they did not
present.
Requirements: Each presenting student should produce one original figure to
contribute to the poster. Can change someone else’s figure to look better, like in the
example on the Results slide. Design a flow chart, no minute details. Talk about the
experimental design and whatever they are doing. Materials: figure of experimental
design. Flow chart. No minute details. She did include what they were treated with
though and that. Talk about experimental design and WHY they are doing whatever
they are doing. Conclusion: may be better to use a summary figure. Markings: group
gets the same; unless someone did not engage properly or did not present.
Data Analysis 10/2/2021
Scientific Method: By observing and taking measurements. By asking questions about
the observations and discovering problems. By formulating a hypothesis – a statement
that tries to explain a problem and that can be tested. By making predictions – stating
what would happen if the hypothesis were true. By testing – to test a hypothesis we
usually carry out controlled experiment(s) that will produce results to either support or
contradict the hypothesis. By interpreting the experimental results (data) objectively and

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