Summary
Summary Mass spectrometry
- Institution
- University Of East Anglia
Drug design and mechanisms of drug action - 1st semester Includes bullet points, key diagrams and images
[Show more]
Seller
Follow
Content preview
Mass SpectrometryMS
Measures mass to charge ratio (m/z)
Biotechnology- analyse proteins and peptides
Pharmaceuticals- drug discovery and drug metabolism
Clinical- haemoglobin analysis and drug testing
Measure mass of molecule as an ion (most cation but sometimes anion)
Large Biomolecules
Mass accuracy of 0.01% of the total mass
Can detect minor mass changes like amino acids
Small Organic Molecules
Mass of molecule can be measured within an accuracy of 5ppm (0.0005%) so can confirm
molecular formula
Structural information generated by fragmentation
MS Instruments
Ion source- ionises sample in gas phase
Mass analyser- sorts ions by mass using
electromagnetic field
Detector- measures indicator quantity and calculates
abundance
Need a vacuum so ions don’t collide
Ionisation- electron impact (EI) harsh, electrospray
ionisation (ESI soft), chemical ionisation (CI soft) or
MALDI (large molecules)
Electrospray Ionisation
MS used as a detector in
chromatography
Problem- need to remove huge excess
of matter used into LC and GC
GC evolved to use narrow capillary
tube
LC is more difficult as it creates huge
volumes of gas which must be
removed
ESi Spectrum
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller alexd1. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £2.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75632 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy revision notes and other study material for 14 years now