Microscopy
Types of Microscope
Optical Laser Scanning Electron
Lens, mirrors and light to Laser light to scan specimens Beams of fast traveling
magnify images and create an image electrons scan a specimen
and are focused onto a plate
to generate an image
Relatively cheap, easy to use More expensive and require Very large and expensive and
and portable training require training
Lowest magnification (x2000) High resolution shows high Highest magnification
and resolution (200nm) contrast (x200,000) and resolution
(0.5nm)
Whole organisms and some Whole organisms or internal Internal cells and organelles
internal cells cells (cant use live organisms)
Used in schools and Used in medical industry Used for bio-medical
fieldwork research
Vacuum is not required Vacuum is not required Vacuum is required
Magnification
Actual size = Image Size ÷ Magnification
I
Magnification = Image Size ÷ Actual Size
Image size = Actual Size x Magnification A M
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