AAB MT Microbiology (Questions And
Answers From The AAB PER Q&A Book)
What term describes the morphology of organisms that vary in shape and
size? Correct Ans - Pleomorphic
Capsules are the basis of which test? Correct Ans - Serotyping
A mucoid colony on blood agar medium indicates that which of the following
characteristics may be present? Correct Ans - Organism has a capsule.
What are dark staining granules called? Correct Ans - Metachromatic
Which of the following organisms are non-motile? Correct Ans -
Shigella and Klebsiella
In the bacterial growth cycle, growth ceases because nutrients are exhausted
or toxic metabolic products have accumulated in which phase? Correct
Ans - Stationary phase
Which of the following genera of bacteria form spores? Correct Ans -
Bacillus and Clostridium
What term is used to describe bacteria that grow in the absence of
atmospheric oxygen and obtain oxygen from oxygen-containing compounds?
Correct Ans - Anaerobes
What is the temperature at which bacteria grow best known as? Correct
Ans - Optimum
On blood agar plates a small zone of alpha hemolysis surrounded by a zone of
beta hemolysis after refrigeration is known as what type of hemolysis?
Correct Ans - Alpha-prime hemolysis
What is the most effective method of sterilization? Correct Ans -
Autoclaving
,Which of the following is used for quality control for the dry heat oven?
Correct Ans - Bacillus subtilis
Media that cannot be heated can be sterilized by what alternate method?
Correct Ans - Filtration
Which one of the following disinfectants is the most effective germicide
against bacteria, spores, tubercle bacilli, fungi, and viruses? Correct Ans -
Chlorine
What is a term that describes a process or treatment that renders a medical
device, instrument, or environmental surface safe to handle? Correct Ans
- Decontamination
What color do gram-positive bacteria stain? Correct Ans - Purple
Acid-fast bacteria appear what color microscopically? Correct Ans -
Red
Acid-fast organisms in tissue are best stained by which of the following
methods? Correct Ans - Kinyoun
Which of the following should be used to remove antimicrobials before
culturing? Correct Ans - Antimicrobial removal device
Which statement concerning sodium polyanethol sulfonate (SPS) is NOT true?
Correct Ans - Activates some antimicrobials
What are some functions of sodium polyanethol sulfonate (SPS)? Correct
Ans - Prevents blood from clotting, prevents phagocytosis, and neutralizes
the bactericidal effect of human serum.
What is the most abundant normal flora in throat cultures? Correct Ans -
Alpha-hemolytic streptococci
Why is it recommended to stab the blood agar plate when setting up throat
cultures? Correct Ans - Some streptococci may not produce beta
hemolysis under aerobic conditions.
, What is the most common pathogen is throat cultures? Correct Ans -
Streptococcus pyogenes
Nasopharangeal swabs are recommended for the detection of which carriers?
Correct Ans - H. influenzae, N. meningitidis, and B. pertussis
Why is a first morning urine specimen preferred for urine cultures?
Correct Ans - Specimen is more concentrated.
What indicates vaginal or urethral contamination of urine? Correct Ans -
Greater than 10 squamous epithelial cells per hpf.
How are smears of cerebrospinal fluid prepared? Correct Ans -
Centrifuged CSF sediment
Smears of CSF are usually stained with which stain? Correct Ans -
Gram stain
Which of the following tests for bacterial antigen on cultures isolated from
CSF is more sensitive and faster? Correct Ans - Latex agglutination
tests
Which of the following is not the cause of a venereal disease? Correct Ans
- Haemophilus aegyptius
A positive coagulase test is observed for which of the following? Correct
Ans - Clotting of plasma
What is the preferred method of differentiating S. aureus from S. epidermidis?
Correct Ans - Coagulase test
A colony of nonhemolytic, catalase-positive, coagulase negative, GPC is most
likely to be which organism? Correct Ans - Staphylococcus epidermidis
When performing a bacitracin differentiation test for Group A beta-hemolytic
streptococci, disks should be used that have how many units? Correct Ans
- 0.02-0.04 unit
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