Uni 6 Assignment Two: Investigative Project Planning and Analysis
Learning Aim: The primary objective of Uni 6 Assignment Two is to guide students in producing a comprehensive plan for an investigative project based on a previously developed proposal. The assignment aims to develop students' abi...
B: Produce a plan for an investigative project based on the proposal.
Hypothesis: As caffeine concentration increases, the daphnia’s heart rate increases.
Method
Before starting the experiment, caffeine concentrations would be required to be produced the
method for this is:
● Take caffeine powder and then use a spatula to take out the caffeine concentration by 1,
0.1, 0.01 and 0.001 mg/L, use a measuring spoon and measuring cup to ensure
accuracy.
● Add water to the caffeine concentrations until the caffeine powder is fully dissolved in the
water.
Once the caffeine concentrations have been created, the experiment can begin, the method to
complete the experiment is:
● After gently removing one daphnia with a pipette and placing it on a cavity slide, place
the cavity slide under a microscope.
● As much water as possible should be taken out of the Daphnia to prevent contamination
of the caffeine concentrations.
● Add a few drops of the caffeine concentrate to the daphnia using a pipette.
● Once the daphnia's heart is visible under the microscope, begin counting heartbeats with
a counter and a stopwatch within 30 seconds.
● Repeat steps 2-4 until all caffeine concentrations have produced results.
Alternative method if laboratory technicians provide caffeine concentrations.
If a scenario occurs where the caffeine concentrations are prepared by the laboratory
technicians, then it’ll be the same method as the original method but without having the need to
create the caffeine concentrations prior to the experiment.
● After gently removing one daphnia with a pipette and placing it on a cavity slide, place
the cavity slide under a microscope.
● As much water as possible should be taken out of the Daphnia to prevent contamination
of the caffeine concentrations.
● Add a few drops of the caffeine concentrate to the daphnia using a pipette.
● Once the daphnia's heart is visible under the microscope, begin counting heartbeats with
a counter and a stopwatch within 30 seconds.
● Repeat steps 2-4 until all caffeine concentrations have produced results.
Equipment
, -Culture of Daphnia (around 10 due to the possibility of the daphnia dying)
-Microscope
-Paper towels
-Caffeine at different concentrations (1, 0.1, 0.01 and 0.001 mg/L)
-Small pipettes
-Beakers
-Measuring cylinder
-Cavity slides
-Cotton wools
-Caffeine powder
-Lab coat
-Goggles
Risk assessment
Type of hazard Hazard Ways to prevent the hazard
Physical Glassware can break which Handle glassware with care
can cause cuts. and place glassware, caffeine
Spillages of pond water and concentrations and pond
caffeine concentrations. water on a flat surface and
away from the edge of the
table.
Chemical Daphnia's can die due to the Ensure the daphnia is
high concentrations of handled with care and take
caffeine. the daphnia back to its
natural habitat.
Electrical Microscope is electric so can Make sure liquids are away
cause electrical shock if liquid from the microscope and
is near the electrical socket. electrical socket. Handle the
Burns on the hand due to the microscope with care to
heat of the microscope. prevent burns. Ensure the
A possibility for the daphnia's daphnia isn’t under the
to die if exposed to light and microscope for too long.
heat for too long.
COSHH
Control of Substances Hazardous to Health (COSHH) are
regulations that are put in place in order to protect employees from injuries and harm to their
health when they’re required to work with substances that can be harmful if misused. By
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