A revision summary of OCR Biology Nucleotides & Nucleic acids 2.1.3
Made by a student who achieved A* in A level Biology.
Covers all the points within the OCR Biology Specification.
Condenses 3 OCR specification books and class notes.
Nucleic Acids DNA & RNA – polynucleotides from DNA replication – the copying of DNA during interphase.
nucleotides Semi -conservative replication – one old strand, one new stand.
Nucleotide 1. Helicase unwinds and unzips DNA. H bonds between bases are
broken and 2 polynucleotide strands separate.
2. Each original strand acts as a template for formation of new DNA
strands
- Components joined by covalent bond 3. Free DNA nucleotides are activated & P groups added - pair with
Sugar-phosphate back bone complementary bases, forming H bonds
- Nucleotides joined by phosphodiester (covalent) bonds 4. DNA polymerase forms phosphodiester bonds between P & S,
in condensation reaction. (P + 5th C) forming sugar phosphate back bone.
- Only Nucleotides with same sugar can join - Enzyme can only bond at 5’ and move to 3’ end.
- Broken by hydrolysis 5. 2 genetically identical DNA molecules formed. 1 template strand, 1
Polynucleotides new complementary strand.
Deoxyribonucleic acid (DNA) – deoxyribose, Thymine Replication errors – sequences of bases not matched exactly causes
base random, spontaneous mutations
DNA Nucleotide base paring Genetic Code – DNA contains genetic code which codes for synthesis of
- Pyrimidine bases always pair with Purine bases all polypeptides.
- Pyrimidines (single C ring) - Thymine, Cytosine Gene - a sequence of DNA that codes for synthesis of a specific
- Purines (double C ring) - Adenine & Guanine polypeptide. In nucleus so code is conserved/ protected.
- Cystine & Guanine bond by 3 H bonds - Each gene occupies a specific locus (position on chromosome)
- Adenine & Thymine bond by 2 H bonds - Each chromosome consists of 1 molecule of DNA
Double Helix - DNA is wrapped around histone proteins (chromatin)
- Strands are antiparallel – 3’-5’ Triplet code
- Polymer contains lots of info - Genetic code contained in sequence of bases in DNA
- Base sequences used as genetic code - Triplet code – sequence of 3 nucleotides called a codon.
- Double stranded = stable molecule & accurate - Each codon codes for a specific aa
replication Non-overlapping Degenerate code – several codons code for same
Ribonucleic acid (RNA). sugar ribose, Uracil base aa
- Copy of sequence of DNA. - 4 bases, 64 base codons possible (43=64). Excess codons used
- Single stranded in;
- 1 start codon signals start of sequence (AUG)
ATP – universal energy currency - 3 stop codons, do not code for any aa, signal end of sequence
- Present in all organisms & cells. - Only 20 aa & lots more codons. Many codons code for same aa (a
- releases energy in small/ manageable quantities degenerate code).
Formation Mutation – changes triplet code which incorporates different aa.
- ATP formed in condensation reaction. ADP + Pi
Alters structure of protein (active site) & protein is non-functional.
ATP
- Hydrolysis ATP ADP + Pi releases energy
- Pi used in bond formation to release lots of energy.
Properties:
- Soluble
- releasing energy in small quantities (no wastage)
- unstable phosphate bond easily broken
- Stored as fat; long term energy store, stable
molecule, used for insulation.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller hyaldridge. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £5.49. You're not tied to anything after your purchase.